Accumulation and sub cellular distribution of lead (Pb) in industrial hemp grown in Pb contaminated soil
Accumulation and sub cellular distribution of lead (Pb) in industrial hemp grown in Pb contaminated soil
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铅污染土壤中工业大麻中铅（Pb）的积累和亚细胞分布
DOI：
10.1016/j.indcrop.2020.113220
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发表时间：
2021-03
影响因子：
5.9
通讯作者：
许艳萍
中科院分区：
文献类型：
--
作者：
许艳萍
工业大麻具有优异的铅（Pb）耐受性和积累能力。进一步提高工业大麻对铅的耐性和富集能力，对工业大麻在植物修复中的应用具有重要意义。采用盆栽试验，研究了工业大麻不同器官中铅的积累规律及其在叶片中的亚细胞分布和可提取态。结果表明，在铅胁迫下，植物体内铅含量随土壤铅浓度的增加而显著增加。铅主要集中在根中，其次是茎和叶，而种子中铅含量最低（<12 mg/kg）。根中铅含量是茎、叶和纤维中铅含量的2-7倍，是种子中铅含量的6-25倍。在亚细胞水平上，工业大麻叶片中Pb主要分布在细胞壁和叶绿体中，Pb含量随土壤Pb浓度的增加先增加后降低，分别在4000和3000 mg/kg时达到最大值。铅在细胞的液泡和可溶性部分中积累较少，在细胞器中积累最少。随着土壤铅浓度的增加，这些亚细胞部位的铅含量没有显著增加。不同铅浓度处理影响大麻叶片中铅的提取形态。Pb在叶片中主要以低活性的HCl提取态存在。植物各部位各形态铅的含量比例不同。叶中各形态Pb含量为Pb HCl（盐酸）>Pb E（乙醇）>Pb W（去离子水）>Pb HAc（乙酸）>Pb NaCl（氯化钠），表明工业大麻中Pb主要以不溶性草酸盐沉淀形式存在，从而减轻了Pb的毒性。该研究结果为工业大麻修复铅污染土壤提供了科学依据。
Industrial hemp has an excellent lead (Pb) tolerance and accumulation capacity. Further enhancing the Pb tolerance and its accumulation in industrial hemp is of great significance for its future application in phytoremediation. In present study, a pot experiment using Pb-contaminated soil was conducted to investigate the accumulation of Pb contents in various organs of industrial hemp and its subcellular distribution and extractable form in the leaves. It was found that under Pb stress, the Pb content in plants increased significantly with an increase in the soil Pb concentration. Lead was mainly concentrated in the roots, followed by stems and leaves, while the lowest Pb contents were found in seeds (<12 mg/kg). The Pb content in roots was 2–7 times higher than in stems, leaves, and fibers, and 6–25 times higher than in seeds. At the subcellular level, Pb in industrial hemp leaves was mainly distributed in the cell wall and chloroplasts, with the Pb content first increasing and then decreasingas the soil Pb concentration increased, reaching a maximum at 4000 and 3000 mg/kg, respectively. Lead was less accumulated in the vacuoles and soluble parts of cells, with the least accumulation occurring in the organelles. There was no nsignificant increase in Pb content in these subcellular locations with the increase in soil Pbconcentration. Treatments with different Pb concentrations affected the extractable form of Pb in hemp leaves. The HCl extractable form of Pb, with low activity, was predominant in the leaves. The proportional content of Pb in each form was different in the various parts of the plant. The Pb content of each form in the leaf was Pb HCl(hydrochloric acid)>Pb E (ethanol)>Pb W (deionized water)>Pb HAc (acetic acid) >Pb NaCl (sodium chloride), indicating that Pb in industrial hemp mainly exists in the form of insoluble oxalate precipitates, thereby alleviating the toxicity of Pb. The results of this study provide a scientific reference for the use of industrial hemp to remediate Pb polluted soils.
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DOI：
10.3923/rjet.2011.162.179
发表时间：
2011-03
期刊：
Research Journal of Environmental Toxicology
影响因子：
--
作者：
Suruchi;P. Khanna
通讯作者：
Suruchi;P. Khanna
利用下一代测序对萝卜 (Raphanus sativus L.) 根进行转录组分析并鉴定参与铅 (Pb) 应激反应的基因
影响因子：
3.7
作者：
Wang Y;Xu L;Chen Y;Shen H;Gong Y;Limera C;Liu L
通讯作者：
Liu L
DOI：
10.1111/j.1469-8137.2010.03456.x
发表时间：
2011
期刊：
The New phytologist
影响因子：
--
作者：
Maozhong Zheng;Chao Cai;Ying Hu;Guoxin Sun;Paul N. Williams;H. Cui;Gang Li;F. Zhao;Yong-guan Zhu-Yo
通讯作者：
Maozhong Zheng;Chao Cai;Ying Hu;Guoxin Sun;Paul N. Williams;H. Cui;Gang Li;F. Zhao;Yong-guan Zhu-Yo
影响因子：
7.4
作者：
ALLAN, DL;JARRELL, WM
通讯作者：
JARRELL, WM
稻草生物炭对老化铜污染土壤中作为饲料种植的苎麻 (Boehmeria nivea L.) 生长、抗氧化能力和铜吸收的影响
影响因子：
6.5
作者：
Rehman, Muzammal;Liu, Lijun;Siddique, Kadambot H. M.
通讯作者：
Siddique, Kadambot H. M.