Effect of real food matrix on the behavior and toxicity of TiO2 nanoparticles
Effect of real food matrix on the behavior and toxicity of TiO2 nanoparticles
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DOI：
10.1007/s11051-022-05585-2
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发表时间：
2022-10-01
影响因子：
2.5
通讯作者：
Dudak, Fahriye Ceyda
中科院分区：
文献类型：
--
作者：
Ersoz, Nurdan;Canga, Emine Merve;Dudak, Fahriye Ceyda
据报道，纳米颗粒（NP）与周围环境成分之间的相互作用会影响纳米颗粒的物理和毒理学特性。在本研究中，评估了真实食物基质（牛奶）的存在对“纳米”尺寸 TiO2（30 nm；Ti30 和 100 nm；Ti100）颗粒在体外消化模拟中的行为和毒性的影响。在消化之前，裸露的 Ti30 和 Ti100 均处于高度聚集状态，而与牛奶的相互作用显着降低了聚集体尺寸。这种减少被认为是由主要由酪蛋白组成的蛋白质冠的空间效应引起的。消化后，简化的模拟牛奶超滤缓冲液 (SMUB) 中 Ti30 和 Ti100 的聚集体大小在口服阶段几乎保持不变，而在胃和肠阶段观察到进一步减少。 SMUB 中的 TiO2 NP 不会对细胞活力造成任何差异，而与牛奶相互作用的 TiO2 NP 对细胞活力有不利影响。高 NP 浓度时，ROS 生成显着增加，这对于 Ti30 来说更为明显。牛奶的存在导致 TiO2 NP 的 ROS 生成减少，这对于 Ti30 来说更为明显。这些发现表明，真实食物基质的存在对纳米粒子消化时的行为和毒性的重要性。
The interactions between nanoparticles (NPs) and the components of the surrounding environment have been reported to affect the physical and toxicological properties of NPs. In the present study, the effects of the presence of a real food matrix (milk) on the behavior and toxicity of "nano"-sized TiO2 (30 nm; Ti30 and 100 nm; Ti100) particles upon in vitro digestion simulation were evaluated. Before digestion, bare Ti30 and Ti100 were both in a highly aggregated state while the interaction with milk reduced the aggregate sizes, significantly. This reduction is considered to be caused by the steric effect of protein corona, mainly composed of caseins. Upon digestion, the aggregate size of Ti30 and Ti100 in a simplified simulated milk ultrafiltrate buffer (SMUB) remained nearly the same in the oral phase, while a further reduction was observed in gastric and intestinal phases. TiO2 NPs in SMUB did not cause any difference in cell viability while the milk-interacted TiO2 NPs had an adverse effect on cell viability. ROS generation was remarkably increased for high NP concentration which is more pronounced for Ti30. The presence of milk led to a reduction in ROS generation of TiO2 NPs which is more pronounced for Ti30. These findings show the importance of the presence of a real food matrix on the behavior and toxicity of NPs when digested.