Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.
Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers.
复制标题
DOI：
10.1007/978-1-0716-2368-8_20
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
苯乙烯马来酸 (SMA) 和二异丁烯马来酸 (DIBMA) 共聚物的开发提供了传统膜蛋白去垢剂萃取的替代方案。通过插入膜中，这些聚合物可以提取膜蛋白以及由聚（苯乙烯共马来酸酐）衍生物制成的天然纳米盘形式的脂质。与去垢剂溶解不同，膜蛋白可能会失去正确折叠和稳定性所必需的环状脂质，而天然纳米盘允许蛋白质驻留在天然脂质环境中。此外，基于聚合物的纳米圆盘可以使用常见的色谱方法进行纯化，类似于用去污剂溶解纯化建立的方案。在这里，我们描述了使用几种商业共聚物对完整膜蛋白的溶解筛选和纯化。
The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.