Novel preparation method for surfactant-lipase complexes utilizing water in oil emulsions.
Novel preparation method for surfactant-lipase complexes utilizing water in oil emulsions.
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DOI：
10.1002/(sici)1097-0290(19970720)55:2
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发表时间：
1997-07
影响因子：
3.8
通讯作者：
S. Okazaki;N. Kamiya;K. Abe;M. Goto;F. Nakashio
中科院分区：
文献类型：
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作者：
S. Okazaki;N. Kamiya;K. Abe;M. Goto;F. Nakashio
本文提出了一种利用油包水乳液制备表面活性剂-脂肪酶复合物的新方法。为了优化制备条件，我们研究了几个操作参数对表面活性剂-脂肪酶复合物在有机介质中的酶活性的影响。在最佳制备条件下[pH 8-10，有机/水= 90/10（v/v），表面活性剂浓度为10 mM]，该复合物能有效地催化苯甲醇与月桂酸的酯化反应。与天然粉末脂肪酶相比，表面活性剂-脂肪酶复合物的酯化率提高了16倍以上。此外，脂肪酶复合物显示出高的储存稳定性。(c)John Wiley & Sons，Inc. Biotechnol Bioeng 55：455-460，1997.
A novel preparation method for surfactant-lipase complexes has been developed utilizing water in oil emulsions. In order to optimize the preparation conditions, we have investigated the effects of several operational parameters on the enzymatic activity of the surfactant-lipase complexes in organic media. When a nonionic surfactant was employed under optimal preparation conditions [alkaline pH 8-10, organic/aqueous = 90/10 (v/v), concentration of surfactant, 10 mM[, the surfactant-lipase complex efficiently catalyzed the esterification of benzyl alcohol with lauric acid in organic media. The esterification rate of the surfactant-lipase complex was increased over 16-fold relative to the native powder lipase. Furthermore, the lipase complex showed high storage stability. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 455-460, 1997.