Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 microns circle
Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 microns circle
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REP3的特性：酿酒酵母质粒2微米圆稳定繁殖所需的顺式作用位点
DOI：
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发表时间：
1985
影响因子：
5.3
通讯作者：
J. Broach
中科院分区：
文献类型：
--
作者：
M. Jayaram;A. Sutton;J. Broach
酵母质粒2微米的稳定繁殖需要复制起点、命名为REP 3的顺式活性位点和两种质粒编码的蛋白质，其是REP 1和REP 2基因的产物。这三个REP基因座似乎构成了一个分配系统，确保有丝分裂后质粒分子平等地分配给母细胞和子细胞。我们已经将REP 3位点完全定位在62个碱基对单位的五个半直接串联重复序列片段内，该片段与2微米基因组的大型独特区域内的HpaI和AvaI限制性位点接壤。此外，我们发现，重复的元素是功能不同的。只有一个重复序列的子集是必要的，以促进充分的分区活动。其他重复似乎促进质粒转录。这些结果进行了讨论的背景下，涉及滴定的质粒特异性蛋白质的REP 3内的重复元素的质粒拷贝控制模型。
Stable propagation of the yeast plasmid 2 microns requires an origin of replication, a cis-active locus designated REP3, and two plasmid-encoded proteins which are the products of the REP1 and REP2 genes. The three REP loci appear to constitute a partitioning system, ensuring equal distribution of plasmid molecules to mother and daughter cells after mitosis. We have localized the REP3 site completely within a segment of five-and-one-half direct tandem repeats of a 62-base-pair unit, bordered by HpaI and AvaI restriction sites within the large unique region of the 2 microns genome. In addition, we find that the repeated elements are functionally distinct. Only a subset of the repeats is necessary to promote full partitioning activity. The other repeats appear to promote plasmid transcription. These results are discussed in the context of a model of plasmid copy control involving titration of a plasmid-specific protein by the repeated elements within REP3.
质粒 P1 复制：重复 DNA 序列的阴性对照。
DOI：
10.1073/pnas.81.20.6456
发表时间：
1984
影响因子：
11.1
作者：
Chattoraj,D;Cordes,K;Abeles,A
通讯作者：
Abeles,A
影响因子：
5.6
作者：
STJOHN, TP;DAVIS, RW
通讯作者：
DAVIS, RW