IDENTIFICATION OF FTSW AND CHARACTERIZATION OF A NEW FTSW DIVISION MUTANT OF ESCHERICHIA-COLI
IDENTIFICATION OF FTSW AND CHARACTERIZATION OF A NEW FTSW DIVISION MUTANT OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.176.23.7140-7147.1994
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发表时间：
1994-12-01
影响因子：
3.2
通讯作者：
DONACHIE, WD
中科院分区：
文献类型：
--
作者：
KHATTAR, MM;BEGG, KJ;DONACHIE, WD
ftsW基因的产物已被鉴定为一种多肽，与相关的RodA蛋白一样，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上显示出异常的迁移率。FtsW以低水平产生，可通过改变mRNA的翻译起始区来增加。FtsW的过量产生强烈抑制细胞生长。一个新的突变等位基因ftsW 201在细胞分裂起始阶段引起温度依赖性阻滞，这与ftsZ突变体中的分裂阻滞相似。ftsW 201等位基因中分裂起始的阻断被证明与FtsZ或FtsZ抑制剂苏拉无关。此外，ftsW 201突变体在允许温度下对分裂起始蛋白FtsZ的过量产生过敏。我们的研究结果表明，FtsW在分裂的早期阶段，这可能涉及与FtsZ的相互作用的作用。
The product of the ftsW gene has been identified as a polypeptide that, like the related RodA protein, shows anomalous mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FtsW is produced at low levels that can be increased by altering the translation initiation region of the mRNA. Overproduction of FtsW strongly inhibits cell growth. A new mutant allele, ftsW201, causes a temperature dependent block in the initiation stage of cell division which is similar to the division block in ftsZ mutants. The block in initiation of division in the ftsW201 allele is shown to be independent of FtsZ or the FtsZ inhibitor, SulA. In addition, the ftsW201 mutant is hypersensitive to overproduction of the division initiation protein FtsZ at the permissive temperature. Our results suggest a role for FtsW in an early stage of division which may involve an interaction with FtsZ.