Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis
Increasing the expression of microRNA-126-5p in the temporal muscle can promote angiogenesis in the chronically ischemic brains of rats subjected to two-vessel occlusion plus encephalo-myo-synangiosis
复制标题
增加颞肌中 microRNA-126-5p 的表达可促进两血管闭塞加脑肌联结大鼠慢性缺血脑中的血管生成
DOI：
10.18632/aging.103431
复制
发表时间：
2020-07-15
期刊：
影响因子：
5.2
通讯作者：
Guo, Ying
中科院分区：
文献类型：
--
作者：
Chen, Chuan;Ling, Cong;Guo, Ying
背景：miR-126-5p在促进内皮细胞（EC）增殖中起重要作用。因此，我们探索miR-126-5p是否可以促进慢性缺血性脑（cib）的EC增殖和血管生成。结果：烟雾病患者血运重建的改善与TM和DM中miR-126-5p表达上调相关。体外实验表明，miR-126-5p通过PI3K/Akt通路促进EC增殖。与对照CIBs相比，agomir组CIBs中p-Akt、VEGF、CD31和eNOS的表达显著增加。与对照组相比，agomir组的ICBP和RCF明显改善。结论：增高TM中miR-126-5p的表达可通过PI3K/Akt通路促进2VO+EMS大鼠CIBs中EC的增殖和血管生成。方法：我们评估烟雾病患者颞肌（TM）和硬脑膜（DM）中miR-126-5p表达与血运重建的相关性。体外研究miR-126-5p对EC增殖及下游信号通路的影响。我们建立双血管闭塞合并脑肌合症（2VO+EMS）动物模型，用miR-126-5p阿戈米尔/安塔戈米尔转染TM，比较各组脑组织中miR-126-5p及相关下游细胞因子的表达，探讨脑血流灌注（ICBP）改善和认知功能（RCF）恢复情况。
Background: miR-126-5p plays an important role in promoting endothelial cell (EC) proliferation. We thus explored whether miR-126-5p can promote EC proliferation and angiogenesis in chronically ischemic brains (CIBs). Results: Improved revascularization in moyamoya patients was correlated with upregulated miR-126-5p expression in the TM and DM. In vitro experiments showed that miR-126-5p promoted EC proliferation through the PI3K/Akt pathway. CIBs from the agomir group exhibited significantly higher p-Akt, VEGF, CD31 and eNOS expression compared with the control CIBs. The ICBP and the RCF were significantly better in the agomir compared with the control group. Conclusion: Increasing miR-126-5p expression in the TM can promote EC proliferation and angiogenesis in CIBs of 2VO+EMS rats through the PI3K/Akt pathway. Methods: We assessed the correlation between revascularization and miR-126-5p expression in the temporal muscle (TM) and dura mater (DM) of moyamoya patients. The effect of miR-126-5p on EC proliferation and downstream signaling pathways was explored in vitro. We established an animal model of two-vessel occlusion plus encephalo-myo-synangiosis (2VO+EMS), transfected the TM with miR-126-5p agomir/antagomir, compared the expression of miR-126-5p and relevant downstream cytokines in brain tissue among different groups, and investigated the improvement in cerebral blood perfusion (ICBP) and the recovery of cognitive function (RCF).