Immunologic trapping in supported liquid membrane extraction
Immunologic trapping in supported liquid membrane extraction
复制标题
支持液膜提取中的免疫捕获
DOI：
10.1021/ac0005013
复制
发表时间：
2000
影响因子：
7.4
通讯作者：
Jenny Emnéus
中科院分区：
文献类型：
--
作者：
E. Thordarson;J. Jönsson;Jenny Emnéus
为了获得高选择性的样品制备，支持液膜（SLM）萃取与免疫识别相结合。SLM采用疏水性聚合物来支持有机溶剂的固定，从而形成无孔膜。所述膜将一侧（供体）的含水样品与另一侧（受体）的接收水相分离。萃取涉及中性化合物在沿着膜连续泵送的样品溶液和膜之间的分配。从膜中，反萃取发生在含有对目标化合物特异性的抗体的第二水相中。因此，在样品制备（ImmunoSLM）的核心是抗体-抗原复合物的形成。当免疫复合物形成时，抗原不能再溶解在有机膜中，从而被捕获在受体中。因此，膜上游离抗原的浓度梯度理想地不受影响，这是该过程的驱动力。在抗体过剩的情况下，接收相中分析物的浓度将容易超过初始样品浓度。在这项工作中，如此形成的免疫复合物进行了定量在线，使用荧光素流动免疫测定在顺序注射分析（SIA）设置。概述的ImmunoSLM-SIA计划被成功地应用于从加标水溶液以及从加标废水样品中提取4-硝基苯酚，表明免疫提取可以是合适的，当处理困难的矩阵。
To obtain a high degree of selectivity in sample preparation, supported liquid membrane (SLM) extraction was combined with immunologic recognition. The SLM employs a hydrophobic polymer for supporting the immobilization of an organic solvent, thus forming a nonporous membrane. Said membrane separates the aqueous sample on one side (donor) from a receiving aqueous phase on the other (acceptor). The extraction involves the partitioning of neutral compounds between the sample solution, continuously pumped alongside the membrane, and the membrane. From the membrane, reextraction takes place into a second aqueous phase containing antibodies specific for the target compound(s). Hence, there is a formation of an antibody-antigen complex at the heart of the sample preparation (ImmunoSLM). When the immunocomplex forms, the antigen can no longer redissolve in the organic membrane, thus being trapped in the acceptor. Consequently, the concentration gradient of free antigen over the membrane is ideally unaffected, this being the driving force for the process. With a surplus of antibody, the concentration of analyte in the receiving phase will easily exceed the initial sample concentration. In this work, the so formed immunocomplex was quantified on-line, using a fluorescein flow immunoassay in a sequential injection analysis (SIA) setup. The outlined ImmunoSLM-SIA scheme was successfully applied for the extraction of 4-nitrophenol from spiked water solutions as well as from a spiked wastewater sample, indicating that the immunoextraction can be suitable when dealing with difficult matrixes.