OsGLU1, A Putative Membrane-bound Endo-1,4-ß-D-glucanase from Rice, Affects Plant Internode Elongation
OsGLU1, A Putative Membrane-bound Endo-1,4-ß-D-glucanase from Rice, Affects Plant Internode Elongation
复制标题
DOI：
10.1007/s11103-005-2972-x
复制
发表时间：
2005
影响因子：
5.1
通讯作者：
Hua-Lin Zhou;Si-Jie He;Yang Cao;Tao Chen;B. Du;C. Chu;Jin‐Song Zhang;S. Chen
中科院分区：
文献类型：
--
作者：
Hua-Lin Zhou;Si-Jie He;Yang Cao;Tao Chen;B. Du;C. Chu;Jin‐Song Zhang;S. Chen
从T-DNA标记的水稻群体中筛选到一株矮秆型粘粒。对Glu突变体T1代的遗传分析表明，野生型与矮秆型的分离比例为3：1，表明该突变体是由一个隐性核位点控制的。突变基因OsGLU 1编码一种膜结合型内切1，4-β-D-葡聚糖酶，该酶在单子叶植物和双子叶植物中高度保守。OsGLU 1基因突变后，细胞伸长降低，纤维素含量降低，果胶含量增加，表明OsGLU 1基因影响水稻节间伸长和细胞壁成分。携带OsGLU 1基因的转基因glu突变体补充了突变并显示野生型表型。此外，OsGLU 1 RNAi植株表现出与glu突变体相似的表型。这些结果表明OsGLU 1在植物细胞生长中起重要作用。赤霉素和油菜素内酯诱导OsGLU 1表达。在水稻基因组中，内切1，4-β-D-葡聚糖酶基因组成了一个15个成员的多基因家族，每个基因在不同的器官中可能有不同的表达模式。这些结果表明，内切-1，4-β-D-葡聚糖酶在水稻生长发育过程中可能起着多种作用。
A dwarf mutantgluwas identified from screening of T-DNA tagged rice population. Genetic analysis of the T1 generation ofglurevealed that a segregation ratio of wild-type:dwarf phenotype was 3:1, suggesting that the mutated phenotype was controlled by a single recessive nuclear locus. The mutated geneOsGLU1, identified by Tail-PCR, encodes a putative membrane-bound endo-1,4-β-D-glucanase, which is highly conserved between mono- and dicotyledonous plants. Mutation of OsGLU1 resulted in a reduction in cell elongation, and a decrease in cellulose content but an increase in pectin content, suggesting thatOsGLU1affects the internode elongation and cell wall components of rice plants. Transgenicglumutants harboring theOsGLU1gene complemented the mutation and displayed the wild-type phenotype. In addition,OsGLU1RNAi plants showed similar phenotype as the glu mutant has. These results indicate that OsGLU1 plays important roles in plant cell growth. Gibberellins and brassinosteroids inducedOsGLU1expression. In rice genome, endo-1,4-β-D-glucanases form a multiple gene family with 15 members, and each may have a distinct expression pattern in different organs. These results indicate that endo-1, 4-β-D-glucanases may play diverse roles in growth and developmental process of rice plants.