The relation between spike-timing dependent plasticity and Ca2+ dynamics in the hippocampal CA1 network
The relation between spike-timing dependent plasticity and Ca2+ dynamics in the hippocampal CA1 network
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DOI：
10.1016/j.neuroscience.2006.11.025
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发表时间：
2007-03-02
期刊：
影响因子：
3.3
通讯作者：
Tsukada, M.
中科院分区：
文献类型：
--
作者：
Aihara, T.;Abiru, Y.;Tsukada, M.
我们在前期的研究中，利用光学成像技术研究了大鼠海马脑片CA1区锋电位时间依赖性突触可塑性（STDP）。结果表明，STDP的轮廓可以分为两种类型，这取决于层特定的位置沿着枝晶。第一个特征是在放射层（SR）的近端区域观察到一个对称的时间窗，第二个特征是在SR的远端区域观察到一个不对称的时间窗。我们的方法涉及到bicuculline（GABA（A）受体拮抗剂）的海马脑片浴应用，这表明GABA能中间神经元的投射是负责的时间窗的对称性。在这项研究中，海马CA1区神经元的细胞内Ca 2+的增加，诱导的协议之间的时间突触前和突触后兴奋（即STDP协议），空间测量通过使用光学成像，以调查如何触发STDP是依赖于细胞内钙离子浓度。我们发现，STDP的大小是密切相关的钙离子增加率（“速度”）的钙瞬变过程中的应用诱导刺激。位置依赖性也进行了分析方面的Ca2+流入。此外，它表明，在应用STDP诱导的刺激过程中的Ca 2+动力学的衰减时间常数也与STDP显着相关。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
In our previous study, spike timing dependent synaptic plasticity (STDP) was investigated in the CA1 area of rat hippocampal slices using optical imaging. It was revealed that the profiles of STDP could be classified into two types depending upon layer specific location along the dendrite. The first was characterized by a symmetric time window observed in the proximal region of the stratum radiatum (SR), and the second by an asymmetric time window in the distal region of the SR. Our methods involved the bath-application of bicuculline (GABA(A) receptor antagonist) to hippocampal slices, which revealed that GABAergic interneuron projections were responsible for the symmetry of a time window. In this study, the intracellular Ca2+ increase of hippocampal CA1 neurons, induced by the protocol of timing between pre- and post-synaptic excitation (i.e. STDP protocol), was measured spatially by using optical imaging to investigate how the triggering of STDP is dependant on intracellular calcium concentration. We found that the magnitude of STDP was closely related to the rate of Ca2+ increase ("velocity") of calcium transient during application of induction stimuli. Location dependency was also analyzed in terms of Ca2+ influx. Furthermore, it was shown that decay time constant of Ca2+ dynamics during the application of STDP-inducing stimuli was also significantly correlated with STDP. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.