Monoclonal Antibody Immunoblot Procedure for Detecting Neotyphodium Coenophialum in Seedling Tall Fescue
Monoclonal Antibody Immunoblot Procedure for Detecting Neotyphodium Coenophialum in Seedling Tall Fescue
复制标题
单克隆抗体免疫印迹法检测高羊茅幼苗中的 Neotyphodium Coenophialum
DOI：
10.1007/978-1-4899-0271-9_47
复制
发表时间：
1997
期刊：
影响因子：
1.5
通讯作者：
J. Bouton
中科院分区：
文献类型：
--
作者：
E. E. Hiatt;N. Hill;J. Bouton
在美国，高羊茅（Festuca arundinacea Schreb.）草坪和饲料品种被吹捧为“增强内生菌”或“无内生菌”。新兴技术表明，随着内生菌感染的无毒种质的产生，了解种子批次的感染状态对于饲料品种将变得越来越重要。种子检测服务通常通过从叶鞘剥离内表皮和包覆的皮质细胞并对组织进行组织学染色以进行显微镜分析来检测内生菌的存在。该过程繁琐且耗时，并且种子需要 4 至 8 周的时间才能生长。因此，需要一种快速、廉价且可重复的技术来评估高羊茅是否存在内生真菌 Neotyphodium coenophialum (Morgan-Jones 和 Gams) Glenn、Bacon 和 Hanlin，comb。十一月本研究的目的是开发一种组织免疫印迹程序，使用针对 N. coenophialum 菌丝蛋白开发的单克隆抗体，检测高羊茅植物和种子中 N. coenophialum 的存在。
In the United States, tall fescue (Festuca arundinacea Schreb.) turf and forage cultivars are being touted as “endophyte enhanced” or “endophyte-free”. Emerging technology suggests that knowledge of infection status of seed lots will become increasingly important for forage cultivars as endophyte-infected non-toxic germplasms are produced. Seed testing services routinely test for presence of endophyte by peeling inner epidermal and subtending cortical cells from the leaf sheath and histologically staining the tissue for microscopic analysis. The procedure is tedious and time consuming and requires a grow out of the seed for a period of 4 to 8 weeks. Hence, a rapid, inexpensive, and reproducible technique is needed to evaluate tall fescue as to the presence or absence of the endophytic fungus, Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin, comb. nov. The purpose of this study was to develop a tissue immunoblot procedure using monoclonal antibodies developed against N. coenophialum mycelial protein that detected the presence of N. coenophialum in tall fescue plants and seeds.