The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes.
The exposure of autoantigens by microparticles underlies the formation of potent inflammatory components: the microparticle-associated immune complexes.
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DOI：
10.1002/emmm.201201846
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发表时间：
2013-02
影响因子：
11.1
通讯作者：
Boilard, Eric
中科院分区：
文献类型：
--
作者：
Cloutier, Nathalie;Tan, Sisareuth;Boudreau, Luc H.;Cramb, Catriona;Subbaiah, Roopashree;Lahey, Lauren;Albert, Alexandra;Shnayder, Ruslan;Gobezie, Reuben;Nigrovic, Peter A.;Farndale, Richard W.;Robinson, William H.;Brisson, Alain;Lee, David M.;Boilard, Eric
免疫球蛋白、抗原和补体可以聚集形成免疫复合物（IC）。ic可能是有害的，因为它们在自身免疫性疾病中传播炎症。与ic一样，亚微米细胞外囊泡称为微颗粒（MP）存在于自身免疫性关节炎患者的滑液中。我们使用高灵敏度流式细胞术和电子显微镜检查类风湿关节炎（RA）的MPs。我们发现RA滑液中的MPs在大小上是高度不均匀的。观察到的较大的MPs实际上是含有mp的ic (mpICs)，占可检测到的ic的大部分。这些mpICs经常表达整合素CD41，与血小板起源一致。尽管血小板来源的MPs表达Fc受体Fcγ riia，但我们发现mpICs的形成独立于该受体。相反，mpICs显示自身抗原vimentin和纤维蛋白原，抗瓜氨酸化肽抗体识别这些靶标有助于mpICs的产生。在功能上，血小板mpICs具有高度的促炎作用，可诱导中性粒细胞产生白三烯。综上所述，我们的数据表明血小板MPs作为自身抗原表达元素具有独特的作用，能够使炎症性ic的形成永久化。
Immunoglobulins, antigens and complement can assemble to form immune complexes (IC). ICs can be detrimental as they propagate inflammation in autoimmune diseases. Like ICs, submicron extracellular vesicles termed microparticles (MP) are present in the synovial fluid from patients affected with autoimmune arthritis. We examined MPs in rheumatoid arthritis (RA) using high sensitivity flow cytometry and electron microscopy. We find that the MPs in RA synovial fluid are highly heterogeneous in size. The observed larger MPs were in fact MP-containing ICs (mpICs) and account for the majority of the detectable ICs. These mpICs frequently express the integrin CD41, consistent with platelet origin. Despite expression of the Fc receptor FcγRIIa by platelet-derived MPs, we find that the mpICs form independently of this receptor. Rather, mpICs display autoantigens vimentin and fibrinogen, and recognition of these targets by anti-citrullinated peptide antibodies contributes to the production of mpICs. Functionally, platelet mpICs are highly pro-inflammatory, eliciting leukotriene production by neutrophils. Taken together, our data suggest a unique role for platelet MPs as autoantigen-expressing elements capable of perpetuating formation of inflammatory ICs.
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影响因子：
6.7
作者：
Connor, David Ewan;Exner, Thomas;Joseph, Joanne Emily
通讯作者：
Joseph, Joanne Emily
影响因子：
3.6
作者：
Flamand, N;Surette, ME;Borgeat, P
通讯作者：
Borgeat, P
炎性关节炎需要中性粒细胞的白细胞B4。
DOI：
10.1084/jem.20052371
发表时间：
2006-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
DOI：
10.4049/jimmunol.0901639
发表时间：
2010-01-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ho PP;Lee LY;Zhao X;Tomooka BH;Paniagua RT;Sharpe O;BenBarak MJ;Chandra PE;Hueber W;Steinman L;Robinson WH
通讯作者：
Robinson WH
影响因子：
27.4
作者：
Biro, Eva;Nieuwland, Rienk;Hack, C. Erik
通讯作者：
Hack, C. Erik