Adhesion proteins for a tight neuron–electrode contact
Adhesion proteins for a tight neuron–electrode contact
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用于紧密神经元-电极接触的粘附蛋白
DOI：
10.1016/s0165-0270(00)00333-2
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发表时间：
2001
影响因子：
3
通讯作者：
L. Tiefenauer
中科院分区：
文献类型：
--
作者：
H. Sorribas;D. Braun;L. Leder;P. Sonderegger;L. Tiefenauer
神经细胞黏附分子Axin-1和NgCAM已经被基因工程改造，并以正确的方向固定在玻璃和二氧化硅表面。用这些黏附分子处理的表面作为培养背根神经节神经元的基质。用荧光干涉显微镜观察神经元细胞膜与表面之间的裂隙。为了进行比较，测量了层粘连蛋白、RGDC、聚赖氨酸和氨基末端表面上的细胞-材料距离。当神经元生长在轴索素-1上时，细胞表面的距离最小(37 Nm)，可能是因为糖萼阻碍了更紧密的接触。细胞外电极的选择性处理可以用来改善细胞与材料的接触，从而增加细胞外记录的信号。
The neural cell adhesion molecules axonin-1 and NgCAM have been genetically engineered and covalently immobilized on glass and silicon oxide surfaces in their correct orientation. Surfaces treated with these adhesion molecules were used as substrates for culturing dorsal root ganglion neurons. The cleft between the neuron cell membrane and the surface was determined using fluorescence interference contrast (FLIC) microscopy. For comparison, cell–material distances on laminin, RGDC, polylysine and amino-terminated surfaces were measured. When the neurons grow on axonin-1 the cell–surface distance is at a minimum (37 nm) probably because the glycocalyx hinders a closer contact. A selective treatment of extracellular electrodes with axonin-1 could be used to improve the cell–material contact and thus increase extracellularly recorded signals.
影响因子：
2.9
作者：
MASSIA, SP;HUBBELL, JA
通讯作者：
HUBBELL, JA