Photoinduced Fluorescence Activation and Nitric Oxide Release with Biocompatible Polymer Nanoparticles
Photoinduced Fluorescence Activation and Nitric Oxide Release with Biocompatible Polymer Nanoparticles
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DOI：
10.1002/chem.201202845
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发表时间：
2012-12-01
影响因子：
4.3
通讯作者：
Sortino, Salvatore
中科院分区：
文献类型：
--
作者：
Deniz, Erhan;Kandoth, Noufal;Sortino, Salvatore
提出了一种将荧光团/光色素二元体和一氧化氮光给体封装在环糊精基聚合物超分子组装体中的可行策略。两个光响应客体在其超分子容器内不相互作用，并且可以在光学控制下并行操作。具体来说，二极体允许荧光在微秒时间尺度上进行数百次循环的可逆切换，而光供体使一氧化氮的不可逆释放成为可能。此外，这些超分子组装穿过人类黑色素瘤癌细胞的膜，并在细胞质中运输它们的货物。一种成分的荧光可以使标记细胞可视化，其可切换特性原则上可以用于获得超分辨率图像，而另一种成分释放的一氧化氮会导致显著的细胞死亡。因此，我们构建具有双重功能的生物相容性纳米颗粒的设计逻辑可能演变为实现成像和治疗应用的有价值的光响应探针。
A viable strategy to encapsulate a fluorophore/photochrome dyad and a nitric oxide photodonor within supramolecular assemblies of a cyclodextrin-based polymer in water was developed. The two photoresponsive guests do not interact with each other within their supramolecular container and can be operated in parallel under optical control. Specifically, the dyad permits the reversible switching of fluorescence on a microsecond timescale for hundreds of cycles, and the photodonor enables the irreversible release of nitric oxide. Furthermore, these supramolecular assemblies cross the membrane of human melanoma cancer cells and transport their cargo in the cytosol. The fluorescence of one component allows the visualization of the labeled cells, and its switchable character could, in principle, be used to acquire super-resolution images, while the release of nitric oxide from the other induces significant cell mortality. Thus, our design logic for the construction of biocompatible nanoparticles with dual functionality might evolve into the realization of valuable photoresponsive probes for imaging and therapeutic applications.