The complete mitochondrial genome of Chinese rare minnow, Gobiocypris rarus (Teleostei: Cypriniformes)
The complete mitochondrial genome of Chinese rare minnow, Gobiocypris rarus (Teleostei: Cypriniformes)
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中国稀有米诺鱼Gobicypris rarus（Teleostei：Cypriniformes）的完整线粒体基因组
DOI：
10.3109/19401736.2011.636441
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发表时间：
2011-10-01
影响因子：
--
通讯作者：
Peng, Zuogang
中科院分区：
文献类型：
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作者：
Wang, Jinjin;Li, Ping;Peng, Zuogang
中国特有的稀有鲦鱼——稀有鲫鱼，是我国极具吸引力的水生实验动物。在本研究中，利用长聚合酶链反应（PCR）方法确定了稀有龟的完整线粒体基因组序列。它的长度为16,601 bp，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区，其基因组成和顺序与大多数其他脊椎动物相似。除 8 个 tRNA 和 ND6 基因外，所有其他线粒体基因均在重链上编码。重链的总体碱基组成为 29.5% A、27.6% T、25.7% C 和 17.2% G，轻微 AT 偏差为 57.1%。有10个基因重叠区域，总计27 bp，以及13个基因间间隔区域，总计63 bp。基于完整的线粒体基因组数据，稀有鮈鱼的线粒体基因组序列有助于更好地解决其在鲤科鱼类中的系统发育位置。
The Chinese rare minnow, Gobiocypris rarus, which is endemic to China, is an attractive aquatic laboratory animal in China. In the present study, the complete mitogenome sequence of G. rarus has been determined using long polymerase chain reaction (PCR) method. It was 16,601 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region, the gene composition and order of which are similar to most other vertebrates. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The overall base composition of the heavy strand is 29.5% A, 27.6% T, 25.7% C, and 17.2% G, with a slight AT bias of 57.1%. There are 10 regions of gene overlap totaling 27 bp and 13 intergenic spacer regions totaling 63 bp. The mitogenome sequence of G. rarus could contribute to a better solution of its phylogenetic position within cyprinid fishes based on the whole mitogenomic data.