PAF, rather than histamine, participates in mouse anaphylactic hypotension
PAF, rather than histamine, participates in mouse anaphylactic hypotension
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DOI：
10.1159/000141516
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发表时间：
2008-01-01
期刊：
影响因子：
3.1
通讯作者：
Kurata, Yasutaka
中科院分区：
文献类型：
--
作者：
Shibamoto, Toshishige;Liu, Wei;Kurata, Yasutaka
本文研究了血小板活化因子（PAF）和组胺在卵清蛋白致敏的BALB/c小鼠过敏性低血压中的作用。通过测量体动脉压（Psa）、门静脉压（Ppv）和中心静脉压（Pcv），研究PAF和组胺对血流动力学指标的影响。静脉注射PAF引起双相Psa反应，最初的快速和短暂的下降，其次是显着的低血压，伴随着减少Pcv。组胺仅引起轻度全身性低血压。在高剂量下，两种药物均使Ppv增加约4 cm H2O。注射抗原后，Psa最初略有增加，然后在抗原给药后10分钟从基线94 +/- 1 mmHg降至46 +/- 1 mmHg，Pcv降低2.5 cm H2O。在抗原注射后5分钟，Ppv增加3.5 cm H2O。用CV-6209（PAF受体拮抗剂，1 mg/kg）或苯海拉明（组胺H-1受体拮抗剂，20 mg/kg）预处理显著减弱抗原诱导的Psa降低。CV-6209的抑制作用大于苯海拉明，这两种拮抗剂的组合几乎完全抑制过敏性低血压。相反，抗原诱导的Ppv增加被CV-6209单独减弱，但被苯海拉明增强。结论：在麻醉BALB/c小鼠中，过敏性低血压主要由PAF介导，在较小程度上由组胺介导。版权所有（C）2008 S. Karger AG，巴塞尔。
We determined the roles of platelet-activating factor (PAF) and histamine in anaphylactic hypotension in ovalbumin-sensitized anesthetized BALB/c mice. The effects of PAF and histamine on hemodynamic variables were studied by measuring the systemic arterial (Psa), portal venous (Ppv) and central venous (Pcv) pressures. Intravenous PAF evoked a bi-phasic Psa response, an initial rapid and transient drop followed by marked hypotension, accompanied by a decrease in Pcv. Histamine caused only mild systemic hypotension. Both agents similarly increased Ppv by approximately 4 cm H2O at high doses. After an injection of antigen, Psa initially increased slightly and then decreased from the baseline of 94 +/- 1 mm Hg to 46 +/- 1 mm Hg at 10 min after antigen administration, with Pcv decreasing by 2.5 cm H2O. Ppv increased by 3.5 cm H2O at 5 min after antigen injection. Pretreatment with either CV-6209 (PAF receptor antagonist, 1 mg/kg) or diphenhydramine (histamine H-1 receptor antagonist, 20 mg/kg) significantly attenuated an antigen-induced decrease in Psa. The inhibitory action of CV-6209 was greater than that of diphenhydramine, and the combination of these 2 antagonists almost completely inhibited the anaphylactic hypotension. In contrast, the antigen-induced increase in Ppv was attenuated by CV-6209 alone but augmented by diphenhydramine. It is concluded that anaphylactic hypotension is mainly mediated by PAF and, to a lesser extent, by histamine in anesthetized BALB/c mice. Copyright (C) 2008 S. Karger AG, Basel.