Disruption of a Rice Chloroplast-Targeted Gene OsHMBPP Causes a Seedling-Lethal Albino Phenotype
Disruption of a Rice Chloroplast-Targeted Gene OsHMBPP Causes a Seedling-Lethal Albino Phenotype
复制标题
水稻叶绿体靶向基因OsHMBPPC的破坏导致幼苗致死的白化表型
DOI：
10.1186/s12284-020-00408-1
复制
发表时间：
2020-07-25
期刊：
影响因子：
5.5
通讯作者：
Tao, D. D.
中科院分区：
文献类型：
--
作者：
Liu, X.;Cao, P. H.;Tao, D. D.
背景叶绿体的发育受叶绿体和核编码基因的协调调控。尽管已有报道称许多调控因子参与了叶绿体的发育，但考虑到这一过程的复杂性，新的因素仍有待确定。结果在本研究中，我们鉴定了针对叶绿体的水稻白化苗期突变致死蛋白1(LAS1)形式的4-羟基-3-甲基-2-烯基二磷酸还原酶(OsHMBPP)。LAS1突变导致幼苗白化致死表型。透射电子显微镜显示，LAS1在叶绿体发育早期是有缺陷的。LAS1在叶片中优先表达，这意味着它在叶绿体发育中起着控制作用。在LAS1中，许多叶绿体编码基因的表达水平发生了显著变化。参与Chl生物合成的核编码基因在LAS1中的表达水平也降低了。我们进一步研究了LAS1中的叶绿体RNA编辑，发现四个叶绿体基因的编辑效率发生了明显的变化。与WT相比，LAS1在叶绿体核糖体的生物发生上表现出缺陷。结论LAS1/OsHMBPP3基因在水稻叶绿体早期发育中起重要作用。
Background Chloroplast development is coordinately regulated by plastid- and nuclear-encoding genes. Although many regulators have been reported to be involved in chloroplast development, new factors remain to be identified, given the complexity of this process. Results In this study, we characterized a rice mutantlethal albinic seedling 1(las1)form of a 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (OsHMBPP) that was targeted to the chloroplasts. TheLAS1mutation caused the albino lethal phenotype in seedlings. Transmission electron microscopy indicated thatlas1were defective in early chloroplast development.LAS1is preferentially expressed in leaves, implying its role in controlling chloroplast development. The expression levels of many chloroplast-encoded genes were altered significantly inlas1. The expression levels of nuclear-encoded gene involved in Chl biosynthesis were also decreased inlas1. We further investigated plastidic RNA editing inlas1and found that the edit efficiency of four chloroplast genes were markly altered. Compared with WT,las1exhibited defective in biogenesis of chloroplast ribosomes. Conclusions Our results show thatLAS1/OsHMBPPplays an essential role in the early chloroplast development in rice.