Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling
Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling
复制标题
DOI：
10.1007/s11745-016-4168-3
复制
发表时间：
2016-08-01
期刊：
影响因子：
1.9
通讯作者：
Williamson, David L.
中科院分区：
文献类型：
--
作者：
Dungan, Cory M.;Li, Ji;Williamson, David L.
本研究的目的是确定热量限制对高脂饮食诱导的骨骼肌生长调节因子改变的影响。我们假设热量限制将逆转高脂饮食诱导的肥胖对REDD 1和mTOR相关信号的负面影响。在HF饮食诱导的肥胖的最初8周期间之后，采用热量限制（CR类似于30%），同时小鼠继续消耗低（LF）或高脂肪（HF）饮食8周。骨骼肌的Western分析显示，CR降低了（p < 0.05）肥胖对脂肪生成蛋白SREBP 1的影响。同样，CR降低了（p < 0.05）肥胖相关的mTORC 1和ERK 1/2信号过度激活的影响，达到与LF小鼠相当的水平。CR还降低了（p < 0.05）肥胖诱导的生长负调节因子、REDD 1和裂解的caspase 3的表达。这些发现对使用短期热量限制的肥胖骨骼肌中失调的生长信号传导的可逆性具有影响。
The objective of this study was to establish the impact of caloric restriction on high fat diet-induced alterations on regulators of skeletal muscle growth. We hypothesized that caloric restriction would reverse the negative effects of high fat diet-induced obesity on REDD1 and mTOR-related signaling. Following an initial 8 week period of HF diet-induced obesity, caloric restriction (CR similar to 30 %) was employed while mice continued to consume either a low (LF) or high fat (HF) diet for 8 weeks. Western analysis of skeletal muscle showed that CR reduced (p < 0.05) the obesity-related effects on the lipogenic protein, SREBP1. Likewise, CR reduced (p < 0.05) the obesity-related effects on the hyperactivation of mTORC1 and ERK1/2 signaling to levels comparable to the LF mice. CR also reduced (p < 0.05) obesity-induced expression of negative regulators of growth, REDD1 and cleaved caspase 3. These findings have implications for on the reversibility of dysregulated growth signaling in obese skeletal muscle, using short-term caloric restriction.