Intracellular protein phosphorylation in adherent U937 monocytes mediated by various culture conditions and fibronectin-derived surface ligands.
Intracellular protein phosphorylation in adherent U937 monocytes mediated by various culture conditions and fibronectin-derived surface ligands.
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由各种培养条件和纤连蛋白衍生的表面配体介导的贴壁 U937 单核细胞的细胞内蛋白磷酸化。
DOI：
10.1016/j.biomaterials.2004.04.002
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发表时间：
2005
期刊：
影响因子：
14
通讯作者：
Kao,WeiyuanJohn
中科院分区：
文献类型：
--
作者：
Chen,Xiuxu;Zuckerman,SeanT;Kao,WeiyuanJohn
Macrophages play a central role in the normal healing process after tissue injury and the host response to foreign objects such as biomaterials. The process leading to macrophage adhesion and activation on protein-adsorbed substrates is complex and unresolved. While the use of primary cells offers clinical relevancy, macrophage cell lines offer unique advantages such as availability and relatively homogeneous phenotype as models to probe the molecular mechanism of cell–surface interaction. Our goal was to better characterize the effect of the culture condition and surface-associated ligands on the extent of U937 adhesion. Tyrosine phosphorylation of intracellular proteins was surveyed as a basis to seek a greater understanding of the molecular mechanism involved in mediating U937 adhesion on various ligand-adsorbed surfaces. U937 viability and adhesion on tissue culture polystyrene (TCPS) increased with (i) increasing serum level, (ii) decreasing tyrosine phosphorylation inhibitor AG18 concentration, or (iii) increasing culture time. The adsorption of various adhesion proteins such as fibronectin and peptide ligands (i.e., RGD, PHSRN) on TCPS did not significantly increase the adherent density of U937 when compared with albumin and PBS ligand controls. However, ligand identity and the presence of phorbol myristate acetate dramatically affected the extent (i.e., increase or decrease) and the identity (i.e., molecular weight) of phosphotyrosine proteins in adherent U937 in a time-dependent manner. The extent and identity of phosphotyrosine proteins did not exhibit a clear AG18 dose dependency, rather the level of tyrosine phosphorylation for a distinct group of proteins was either increased or decreased for a given AG18 concentration.
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日本农村地区糖尿病患病率：按 75 克 OGTT 计算
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
S. Takashina;H. Tomaru;K. Komatsu;N. Utsumi;S. Nakashima;S. Yamada;M. Nagashima;T. Murata;W. Koyama
通讯作者：
W. Koyama
10,000 名以色列成年男性糖尿病发病率的变化及其发育相关因素。
DOI：
--
发表时间：
1978
期刊：
Advances in Metabolic Disorders
影响因子：
--
作者：
J. Medalie;J. Herman;U. Golgbourt;C. Papier
通讯作者：
C. Papier
影响因子：
5
作者：
MARMOT, MG;SYME, SL
通讯作者：
SYME, SL
马萨诸塞州公共卫生部。
DOI：
--
发表时间：
1982
期刊：
影响因子：
--
作者：
Lambert Ca;Netherton Dr;Finison Lj;Hyde Jn;Spaight Sj
通讯作者：
Spaight Sj
社会阶层、易感性和疾病。
影响因子：
5
作者：
S. Syme;L. Berkman
通讯作者：
L. Berkman