Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L
Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L
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DOI：
10.1016/j.ijbiomac.2015.03.026
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发表时间：
2015-06-01
影响因子：
8.2
通讯作者：
Sun, Gui-Ju
中科院分区：
文献类型：
--
作者：
Tang, Hua-Li;Chen, Chen;Sun, Gui-Ju
枸杞多糖的分离纯化、性质及降血糖活性研究。在本研究中进行了调查。采用超滤膜分离得到一种水溶性多糖（LBP），经DEAE纤维素柱层析和Sephadex G-150凝胶柱层析得到LBP 3a和LBP 3b。高效渗透色谱（HPGPC）分析表明LBP 3b的平均分子量（Mw）为4.92 kDa。单糖组成分析显示LBP 3b由甘露糖、鼠李糖、葡萄糖、半乳糖和木糖组成，摩尔比为5.52：5.11：28.06：1.00：1.70。采用UV、FT-IR、NMR和SEM对LBP 3b的结构进行了初步表征。体外细胞实验表明，LBP 3b对葡萄糖的吸收有明显的抑制作用，且呈剂量依赖性。该研究表明LBP 3b具有作为抗糖尿病剂的潜在用途。(C)2015 Elsevier B. V.版权所有。
Purification, characterization and hypoglycemic activities of polysaccharide from Lycium barbarum L. were investigated in this study. A water soluble polysaccharide (LBP) was obtained with ultrafiltration membranes separation, which was further purified by chromatography of DEAE cellulose column and Sephadex G-150 to get LBP3a and LBP3b. The high performance permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of LBP3b was 4.92 kDa. Monosaccharide composition analysis revealed that the LBP3b was comprised of mannose, rhamnose, glucose, galactose and xylose with a molar ratio of 5.52:5.11:28.06:1.00:1.70. The preliminary structure features of LBP3b were investigated by UV, FT-IR, NMR and SEM. In vitro cell experiments showed that LBP3b had significantly inhibited the absorption of glucose in a dose-dependent manner. The study showed that LBP3b had potential use as an anti-diabetic agent. (C) 2015 Elsevier B.V. All rights reserved.