A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics
A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics
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DOI：
10.1093/pcp/pci251
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发表时间：
2006-03-01
影响因子：
4.9
通讯作者：
Ezura, H
中科院分区：
文献类型：
--
作者：
Sun, HJ;Uchii, S;Ezura, H
我们报告了一项高效的机构介导的小矮矮番茄（Lycopersicon esculentum）的遗传转化的方案，Micro-Tom，Micro-TOM，这是番茄功能基因组学的模型品种。与农杆菌（根瘤菌放射线杆菌）接种的番茄的子叶外植体C58C1RIF（R）携带二进制载体Pig121Hm的C58C1RIF（R）产生了大量的嵌合非转基因性和转基因的芽。从选择媒体上的一系列不定芽中反复的射击伸长导致产生来自独立转化事件的多个转基因植物。转化效率超过了40％的外植体。该方案可能成为番茄功能基因组学的强大工具。
We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.