Gold nanoparticle-based colorimetric assay for cancer diagnosis
Gold nanoparticle-based colorimetric assay for cancer diagnosis
复制标题
DOI：
10.1016/j.bios.2009.12.022
复制
发表时间：
2010-04-15
影响因子：
12.6
通讯作者：
Katayama, Yoshiki
中科院分区：
文献类型：
--
作者：
Kang, Jeong-Hun;Asami, Yoji;Katayama, Yoshiki
开发了一种新的基于金纳米颗粒（GNP）的比色分析用于癌症诊断。该系统是基于与阳离子蛋白激酶C（PKC）α-特异性肽底物的非交联聚集机制，该底物用作柠檬酸盐包被的GNP的凝结剂，具有阴离子表面电荷。PKC α对肽底物的磷酸化抑制了GNP聚集，导致红色，但在非磷酸化的情况下，GNP溶液的颜色从红色变为蓝色，表明颗粒聚集。此外，从使用癌细胞系或异种移植的小鼠癌症和正常小鼠组织的实验中鉴定了GNP分散体的颜色变化与活化的PKC α水平之间的相关性。当我们的系统应用于人乳腺癌和正常人乳腺组织时，癌组织裂解物变成红色，表明GNP分散，而来自正常组织的所有裂解物使GNP溶液变成蓝色。MALDI-TOF MS分析和Western blotting实验证实，癌组织和正常组织中的这些不同结果反映了PKC α活性的差异。本研究首次报道了基于GNP的比色法在癌症诊断中的应用。(c)2009爱思唯尔有限公司版权所有。
A novel gold nanoparticle (GNP)-based colorimetric assay was developed for cancer diagnosis. This system is based on the noncrosslinking aggregation mechanism with a cationic protein kinase C (PKC) alpha-specific peptide substrate, which is used as a coagulant of citrate-coated GNP with anionic surface charges. The phosphorylation of peptide substrates by PKC alpha suppressed GNP aggregation, resulting in a red color, but in the case of non-phosphorylation, the color of the GNP solution changed from red to blue, indicating particle aggregation. Moreover, a correlation between the color change of the GNP dispersions and the level of activated PKC alpha was identified from experiments using cancer cell lines, or xenografted mouse cancer and normal mouse tissues. When our system was applied to human breast cancers and normal human breast tissues, cancer tissue lysates became red in color, indicating GNP dispersion, while all lysates from normal tissue turned the GNP solution blue. MALDI-TOF MS analysis and Western blotting experiment confirmed that these different results between cancer and normal tissues reflected the difference in PKC alpha activity. This study is the first report on the application of the GNP-based colorimetric assay to the diagnosis of cancer. (c) 2009 Elsevier B.V. All rights reserved.