Tomographic phosphorescence lifetime multiplexing.
Tomographic phosphorescence lifetime multiplexing.
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DOI：
10.1364/ol.43.003104
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发表时间：
2018-07-01
期刊：
影响因子：
3.6
通讯作者：
Hou SS
中科院分区：
文献类型：
--
作者：
Kumar ATN;Hou SS
我们提出了一种断层扫描重建算法，用于使用点或空间图案源和探测器，从时间分辨测量中恢复混浊介质内多种磷光染料的分布。该算法采用时间分辨数据的多指数分析，然后对衰变幅度进行层析成像反演，以恢复介质中存在的每个寿命的独立产量分布。使用蒙特卡罗模拟，我们通过计算证明，与时域磷光的直接反演相比，这种两步反演方法在定量和定位精度方面提供了数倍的提高。我们还展示了嵌入厚组织中多达三个磷光寿命的断层扫描重建。所提出的算法可以实现发光和磷光染料的定量多重断层扫描，用于广泛的体内应用。
We present a tomographic reconstruction algorithm for recovering distributions of multiple phosphorescent dyes within turbid media from time-resolved measurements, using either point or spatially patterned sources and detectors. The algorithm employs a multi-exponential analysis of time-resolved data, followed by tomographic inversion of the decay amplitudes to recover independent yield distributions for each lifetime present in the medium. Using Monte Carlo simulations, we computationally demonstrate that this two-step inversion approach provides several-fold improvement in quantitative and localization accuracy compared to a direct inversion of the time domain phosphorescence. We also demonstrate the tomographic reconstruction of up to three phosphorescent lifetimes embedded in thick tissue. The proposed algorithm can allow quantitative multiplexed tomography of luminescent and phosphorescent dyes for a wide range of in vivo applications.