Nrl-Cre transgenic mouse mediates loxP recombination in developing rod photoreceptors.
Nrl-Cre transgenic mouse mediates loxP recombination in developing rod photoreceptors.
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DOI：
10.1002/dvg.22918
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Chen S
中科院分区：
文献类型：
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作者：
Brightman DS;Razafsky D;Potter C;Hodzic D;Chen S
发育中的小鼠视网膜是研究神经发生和分化的易于处理的模型。虽然存在转基因Cre小鼠系来介导发育中小鼠视网膜的条件遗传操作，但它们中没有一个在早期发育中的视杆细胞中特异性地起作用。对于发育中视网膜的条件遗传操作，我们创建了Nrl-Cre小鼠系，其中Nrl启动子驱动视杆前体中Cre的表达。我们的结果表明，Nrl-Cre表达是特定于视网膜，在视网膜发育过程中，它驱动杆特异性重组与内源性Nrl表达类似的时间模式。这种Nrl-Cre转基因不会对视网膜结构和功能产生负面影响。两者合计，我们的数据表明，Nrl-Cre小鼠系是一个有价值的工具，以驱动铬介导的重组，特别是在发展杆。
The developing mouse retina is a tractable model for studying neurogenesis and differentiation. Although transgenic Cre mouse lines exist to mediate conditional genetic manipulations in developing mouse retinas, none of them act specifically in early developing rods. For conditional genetic manipulations of developing retinas, we created a Nrl-Cre mouse line in which the Nrl promoter drives expression of Cre in rod precursors. Our results show that Nrl-Cre expression is specific to the retina where it drives rod-specific recombination with a temporal pattern similar to endogenous Nrl expression during retinal development. This Nrl-Cre transgene does not negatively impact retinal structure and function. Taken together, our data suggest that the Nrl-Cre mouse line is a valuable tool to drive Cre-mediated recombination specifically in developing rods.