IMMUNOLOCALIZATION OF MATRIX METALLOPROTEINASE-3 (STROMELYSIN) IN RHEUMATOID SYNOVIOBLASTS (B-CELLS) - CORRELATION WITH RHEUMATOID-ARTHRITIS
IMMUNOLOCALIZATION OF MATRIX METALLOPROTEINASE-3 (STROMELYSIN) IN RHEUMATOID SYNOVIOBLASTS (B-CELLS) - CORRELATION WITH RHEUMATOID-ARTHRITIS
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DOI：
10.1136/ard.48.8.645
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发表时间：
1989-08-01
影响因子：
27.4
通讯作者：
NAGASE, H
中科院分区：
文献类型：
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作者：
OKADA, Y;TAKEUCHI, N;NAGASE, H
结缔组织细胞产生的金属蛋白酶可能在类风湿性关节炎关节破坏中发挥关键作用。能够降解软骨蛋白聚糖和 IX 型胶原并激活原胶原酶的基质金属蛋白酶 3（MMP-3；基质溶解素）免疫定位于类风湿滑膜的增生性滑膜衬里细胞中，但不定位于正常滑膜的细胞中。通过免疫电子显微镜观察，负责合成 MMP-3 的细胞具有滑膜母细胞（B 细胞）的表型，但不具有吞噬性滑膜巨噬细胞（A 细胞）的表型。培养的单层类风湿滑膜细胞仅在巨噬细胞条件培养基处理下合成MMP-3。当用单价离子载体莫能菌素处理样本时，MMP-3 在类风湿滑膜和培养的滑膜细胞中的免疫定位是可能的。这些结果表明，MMP-3是由滑膜中存在的活化巨噬细胞衍生的因子刺激的增生性滑膜母细胞连续合成和分泌的，无需储存。
Metalloproteinases produced by connective tissue cells may play a key part in the destruction of joints in rheumatoid arthritis. Matrix metalloproteinase 3 (MMP-3; stromelysin) capable of degrading cartilage proteoglycans and type IX collagen and of activating procollagenase was immunolocalised in hyperplastic synovial lining cells in rheumatoid synovium, but not in the cells of normal synovium. Cells responsible for synthesis of MMP-3 have the phenotype of synovioblasts (B cells) by immunoelectron microscopy, but not of phagocytic synovial macrophages (A cells). Cultured monolayer of rheumatoid synovial cells synthesises MMP-3 only under treatment with macrophage conditioned medium. Immunolocalisation of MMP-3 in rheumatoid synovium and cultured synovial cells was possible when the specimens were treated with a monovalent ionophore, monensin. These results suggest that MMP-3 is synthesized and secreted continuously without storage from hyperplastic synovioblasts stimulated by factor(s) derived from activated macrophages present in the synovium.