New wrinkling substrate assay reveals traction force fields of leader and follower cells undergoing collective migration
New wrinkling substrate assay reveals traction force fields of leader and follower cells undergoing collective migration
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新的起皱底物测定揭示了经历集体迁移的前导细胞和跟随细胞的牵引力场
DOI：
10.1016/j.bbrc.2016.11.142
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发表时间：
2017
影响因子：
3.1
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Yokoyama;S.;Matsui;T.S.;Deguchi;S.
物理力在协调上皮细胞的集体迁移中发挥着至关重要的作用，但这种与力相关的现象的细节仍不清楚，部分原因是缺乏探测潜在力场的稳健方法。在这里，我们开发了一种制造有机硅基底的方法，该方法可以高灵敏度地检测细胞牵引力。具体而言，将有机硅弹性体在加热下暴露于氧等离子体。热量的去除使基底收缩，从而以空间均匀的方式降低其临界屈曲应变。因此，即使是很小的细胞牵引力也可以被视为微皱纹，这些皱纹在与施加的力正交的方向上可逆地出现在基底上。使用这种技术，我们发现 MDCK-II 细胞簇中所谓的领导细胞可以发挥与追随细胞不同的显着牵引力。我们发现牵引力的方向与簇内局部单个细胞的长轴高度相关。这些结果表明，MDCK-II 细胞集体迁移中的力场主要是在单个细胞尺度上局部确定的，而不是在整个细胞簇尺度上全局确定的。
Physical forces play crucial roles in coordinating collective migration of epithelial cells, but details of such force-related phenomena remain unclear partly due to the lack of robust methodologies to probe the underlying force fields. Here we develop a method for fabricating silicone substrates that detect cellular traction forces with a high sensitivity. Specifically, a silicone elastomer is exposed to oxygen plasma under heating. Removal of the heat shrinks the substrate so as to reduce its critical buckling strain in a spatially uniform manner. Thus, even small cellular traction forces can be visualized as micro-wrinkles that are reversibly emerged on the substrate in a direction orthogonal to the applied forces. Using this technique, we show that so-called leader cells in MDCK-II cell clusters exert significant magnitudes of traction forces distinct from those of follower cells. We reveal that the direction of traction forces is highly correlated with the long axis of the local, individual cells within clusters. These results suggest that the force fields in collective migration of MDCK-II cells are predominantly determined locally at individual cell scale rather than globally at the whole cell cluster scale.