α-actinin-2, a cytoskeletal protein, binds to angiogenin
α-actinin-2, a cytoskeletal protein, binds to angiogenin
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DOI：
10.1016/j.bbrc.2005.01.158
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发表时间：
2005-04-08
影响因子：
3.1
通讯作者：
Xu, ZP
中科院分区：
文献类型：
--
作者：
Hu, HJ;Gao, XW;Xu, ZP
血管生成素是一种血管生成因子，参与肿瘤的发生。然而，目前还没有特定的细胞内蛋白与血管生成素直接相互作用。在本研究中，我们通过酵母双杂交筛选鉴定了肌动蛋白交联蛋白α-肌动蛋白-2作为潜在的血管生成素相互作用伙伴。这种相互作用通过不同的方法得到了证实。首先，用Ni2+-琼脂糖树脂将血管生成素与His标记的α-肌动蛋白-2一起拉下。用抗血管生成素单抗将α-肌动蛋白-2与血管生成素进行免疫共沉淀。第三，通过荧光共振能量转移分析揭示了这两种蛋白质在体内的相互作用。由于α-肌动蛋白家族成员在细胞增殖、迁移和侵袭中起关键作用，α-肌动蛋白-2和血管生成素之间的相互作用可能是血管生成素在血管生成中的一个可能机制。我们的发现首次证明了胞浆蛋白与血管生成素的相互作用，这可能是抗血管生成和抗肿瘤治疗的新干扰靶点。(C)2005 Elsevier Inc.保留所有权利。
Angiogenin is an angiogenic factor which is involved in tumorigenesis. However, no particular intracellular protein is known to interact directly with angiogenin. In the present study, we reported the identification of alpha-actinin-2, an actin-crosslinking protein, as a potential angiogenin-interacting partner by yeast two-hybrid screening. This interaction was confirmed by different approaches. First, angiogenin was pulled down together with His-tagged alpha-actinin-2 by Ni2+-agarose resins. Second, alpha-actinin-2 was coimmunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody. Third, the in vivo interaction of these two proteins was revealed by fluorescence resonance energy transfer analysis. Since members of alpha-actinin family play pivotal roles in cell proliferation, migration, and invasion, the interaction between alpha-actinin-2 and angiogenin may underline one possible mechanism of angiogenin in angiogenesis. Our finding presents the first evidence of an interaction of a cytosolic protein with angiogenin, which might be a novel interference target for anti-angiogenesis and anti-tumor therapy. (c) 2005 Elsevier Inc. All rights reserved.