Discrimination of Panax ginseng Roots Cultivated in Different Areas in Korea Using HPLC-ELSD and Principal Component Analysis
Discrimination of Panax ginseng Roots Cultivated in Different Areas in Korea Using HPLC-ELSD and Principal Component Analysis
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DOI：
10.5142/jgr.2011.35.1.031
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发表时间：
2011-03-01
影响因子：
6.3
通讯作者：
Baek, Nam-In
中科院分区：
文献类型：
--
作者：
Lee, Dae-Young;Cho, Jin-Gyeong;Baek, Nam-In
为了区分班乃人参的种植面积，利用 HPLC 获得的定量和定性数据进行主成分分析 (PCA)。建立了一种新的 HPLC 结合蒸发光散射检测 (HPLC-ELSD) 方法，用于同时定量人参根中 10 种主要人参皂苷，即 Rh-1、Rg(2)、Rg(3)、Rg(1)、Rf、Re、Rd、Rb-2、Rc 和 Rb。在碳水化合物分析柱上实现了这十种人参皂苷的同时分离。流动相由乙腈-水-异丙醇和乙腈-水-异丙醇组成，采用梯度洗脱。在韩国江华和丰基两个不同种植区生产的人参根中，发现人参皂苷的质量和数量特征存在明显差异。对通过 HPLC 分析获得的人参皂苷谱进行 PCA 分析。使用两个主成分 (PC) 的 PCA 评分图显示江华和丰吉种植的人参根具有良好的分离性。 PC1 影响分离，捕获 43.6% 的方差，而 PC2 影响分化，解释 18.0% 的方差。贡献最高的成分是PC用人参皂苷Rg3！ PC2 的人参皂苷 Rf。特别是鲜重轻于147克的六年生小参根的PCA评分图显示出更明显的区分度。 PC1 影响不同样本集之间的分离，捕获 51.8% 的方差，而 PC2 影响分化，也解释 28.0% 的方差。对 PCI 贡献最高的成分是人参皂苷 Rf，对 PC2 贡献最高的成分是人参皂苷 Rg(2)。总之，使用碳水化合物柱的 HPLC-ELSD 方法可以同时定量十种主要人参皂苷，并且对 HPLC 色谱图上显示的人参皂苷峰进行 PCA 分析将是区分人参根种植面积的非常可接受的策略。
In order to distinguish the cultivation area of Panay ginseng, principal component analysis (PCA) using quantitative and qualitative data acquired from HPLC was carried out. A new HPLC method coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of ten major ginsenosides, namely Rh-1, Rg(2), Rg(3), Rg(1), Rf, Re, Rd, Rb-2, Rc, and Rb, in the root of P ginseng C. A. Meyer. Simultaneous separations of these ten ginsenosides were achieved on a carbohydrate analytical column. The mobile phase consisted of acetonitrile-water-isopropanol, and acetonitrile-water-isopropanol using a gradient elution. Distinct differences in qualitative and quantitative characteristics for ginsenosides were found between the ginseng roots produced in two different Korean cultivation areas, Ganghwa and Punggi. The ginsenoside profiles obtained via HPLC analysis were subjected to PCA. PCA score plots using two principal components (PCs) showed good separation for the ginseng roots cultivated in Ganghwa and Punggi. PC1 influenced the separation, capturing 43.6% of the variance, while PC2 affected differentiation, explaining 18.0% of the variance. The highest contribution components were ginsenoside Rg3 for PC! and ginsenoside Rf for PC2. Particularly, the PCA score plot for the small ginseng roots of six-year old, each of which was light than 147 g fresh weight, showed more distinct discrimination. PC1 influenced the separation between different sample sets, capturing 51.8% of the variance, while PC2 affected differentiation, also explaining 28.0% of the variance. The highest contribution component was ginsenoside Rf for PCI and ginsenoside Rg(2) for PC2. In conclusion, the HPLC-ELSD method using a carbohydrate column allowed for the simultaneous quantification of ten major ginsenosides, and PCA analysis of the ginsenoside peaks shown on the HPLC chromatogram would be a very acceptable strategy for discrimination of the cultivation area of ginseng roots.