Chemical Reagents for Protein Modification
Chemical Reagents for Protein Modification
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DOI：
10.1201/9780429292873
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Lundblad
中科院分区：
文献类型：
--
作者：
R. Lundblad
化学试剂蛋白质修饰，第2版提供了一个独特的结合理论和实际考虑使用的化学试剂位点特异性修饰的蛋白质。这本书分为三个部分，第一部分描述了一般技术，包括各种修饰反应的有机化学信息;位点特异性修饰蛋白质的分离和表征，包括通过电泳分离蛋白质的应用，然后通过印迹;蛋白质中肽键的特异性化学裂解;通过高效液相色谱和电泳分离肽;以及使用化学试剂评估蛋白质的构象变化。第二部分提供了用于蛋白质中单个氨基酸残基的位点特异性修饰的试剂和反应的简明描述。最后一节介绍了使用化学试剂来标记蛋白质中的生物学重要位点的描述，包括酶活性位点和使用共价交联来测量蛋白质-蛋白质相互作用。特别强调的是使用光亲和试剂。这本书将是一个非常有用的研究工具，为所有研究人员感兴趣的溶液化学的蛋白质。
Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.