Holographic optogenetic stimulation of patterned neuronal activity for vision restoration
Holographic optogenetic stimulation of patterned neuronal activity for vision restoration
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DOI：
10.1038/ncomms2500
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发表时间：
2013-02-01
影响因子：
16.6
通讯作者：
Shoham, Shy
中科院分区：
文献类型：
--
作者：
Reutsky-Gefen, Inna;Golan, Lior;Shoham, Shy
当自然光接收被破坏时，就像视网膜外退行性疾病一样，人工刺激存活的神经细胞提供了一种绕过受损神经回路的潜在策略。近年来，使静止神经元光敏的光敏蛋白为光遗传神经元控制创造了前所未有的机会，激发了光学视网膜假体的早期发展。选择性地刺激大量神经群体对于在大脑中激发有意义的知觉是必不可少的。在这里，我们提供了仿生视觉恢复的全息光刺激策略的第一个演示。在盲人视网膜中，我们展示了可靠的全息图案化光遗传刺激视网膜神经节细胞，具有毫秒的时间精度和细胞分辨率。全息激发策略可以实现对分布式神经元电路的灵活控制，可能为高清晰度视觉恢复设备和更多的医疗和科学神经光子学应用铺平道路。
When natural photoreception is disrupted, as in outer-retinal degenerative diseases, artificial stimulation of surviving nerve cells offers a potential strategy for bypassing compromised neural circuits. Recently, light-sensitive proteins that photosensitize quiescent neurons have generated unprecedented opportunities for optogenetic neuronal control, inspiring early development of optical retinal prostheses. Selectively exciting large neural populations are essential for eliciting meaningful perceptions in the brain. Here we provide the first demonstration of holographic photo-stimulation strategies for bionic vision restoration. In blind retinas, we demonstrate reliable holographically patterned optogenetic stimulation of retinal ganglion cells with millisecond temporal precision and cellular resolution. Holographic excitation strategies could enable flexible control over distributed neuronal circuits, potentially paving the way towards high-acuity vision restoration devices and additional medical and scientific neuro-photonics applications.