Expression of retrovirally transduced genes in primary cultures of adult rat hepatocytes.
Expression of retrovirally transduced genes in primary cultures of adult rat hepatocytes.
复制标题
逆转录病毒转导基因在成年大鼠肝细胞原代培养物中的表达。
DOI：
10.1073/pnas.84.10.3344
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发表时间：
1987
影响因子：
11.1
通讯作者：
Leffert,H
中科院分区：
文献类型：
--
作者：
Wolff,JA;Yee,JK;Skelly,HF;Moores,JC;Respess,JG;Friedmann,T;Leffert,H
用表达人次黄嘌呤/鸟嘌呤磷酸核糖基转移酶或转座子Tn5新霉素抗性基因的逆转录病毒载体感染已分化的大鼠原代肝细胞。在培养第1~5天的短暂细胞复制和短暂去分化过程中，只有在细胞感染后才能检测到这些标志物的表达。在此期间整合的前病毒在随后的整个培养静止期至少25天内仍保持完全表达。用新霉素类似物G418筛选感染新霉素载体的细胞，出现肝细胞形态的细胞，免疫沉淀法检测到新合成的白蛋白，表明肝细胞感染成功。
Differentiated primary rat hepatocyte cultures have been infected with retroviral vectors expressing human hypoxanthine/guanine phosphoribosyltransferase or the transposon Tn5 neomycin-resistance gene. Expression of the markers was detected only after infection of the cells during a short period of cell replication and transient dedifferentiation from days 1 to 5 of culture. Provirus integrated during that period remains fully expressed during the entire subsequent stationary period of culture up to at least 25 days. Selection with the neomycin analogue G418 of cells infected with the neomycin vector led to the appearance of cells with hepatocyte morphology in which newly synthesized albumin was detectable by immunoprecipitation, indicating successful infection of hepatocytes.