A diRNA-protein scaffold module mediates SMC5/6 recruitment in plant DNA repair
A diRNA-protein scaffold module mediates SMC5/6 recruitment in plant DNA repair
复制标题
diRNA-蛋白质支架模块介导植物 DNA 修复中的 SMC5/6 募集
DOI：
10.1093/plcell/koac191
复制
发表时间：
2022-07-01
期刊：
影响因子：
11.6
通讯作者：
Yang, Chengwei
中科院分区：
文献类型：
--
作者：
Jiang, Jieming;Ou, Xiaolin;Yang, Chengwei
The localization of the SMC5/6 complex at double-strand breaks is dependent on the IDN2-CDC5-ADA2b protein scaffold, which is further mediated by DNA damage-induced RNAs in plant cells.In eukaryotes, the STRUCTURAL MAINTENANCE OF CHROMOSOME 5/6 (SMC5/6) complex is critical to maintaining chromosomal structures around double-strand breaks (DSBs) in DNA damage repair. However, the recruitment mechanism of this conserved complex at DSBs remains unclear. In this study, using Arabidopsis thaliana as a model, we found that SMC5/6 localization at DSBs is dependent on the protein scaffold containing INVOLVED IN DE NOVO 2 (IDN2), CELL DIVISION CYCLE 5 (CDC5), and ALTERATION/DEFICIENCY IN ACTIVATION 2B (ADA2b), whose recruitment is further mediated by DNA-damage-induced RNAs (diRNAs) generated from DNA regions around DSBs. The physical interactions of protein components including SMC5-ADA2b, ADA2b-CDC5, and CDC5-IDN2 result in formation of the protein scaffold. Further analysis indicated that the DSB localization of IDN2 requires its RNA-binding activity and ARGONAUTE 2 (AGO2), indicating a role for the AGO2-diRNA complex in this process. Given that most of the components in the scaffold are conserved, the mechanism presented here, which connects SMC5/6 recruitment and small RNAs, will improve our understanding of DNA repair mechanisms in eukaryotes.
