Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses
Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses
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通过血液生化和体液非靶向代谢组分析表征棉酚处理的奶牛的代谢特征
DOI：
10.1021/acs.jafc.7b03544
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发表时间：
2017
影响因子：
6.1
通讯作者：
Zhang Junmin
中科院分区：
文献类型：
--
作者：
Tang Chaohua;Zhang Kai;Zhan Tengfei;Zhao Qingyu;Zhang Junmin
为了研究棉酚对奶牛代谢紊乱的影响，将12头奶牛分为对照组和试验组，每千克干物质饲料中添加1000 mg棉酚，连续28天。乳蛋白和乳糖水平显著降低(分别为3.40%vs3.16%，P=0.044；5.15%vs4.91%，P=0.027)。血浆丙氨酸氨基转移酶(P=0.092)、胆碱酯酶(P=0.02)、谷胱甘肽转移酶(P=0.0005)升高，血糖降低(P=0.076)。基于质谱学的比较代谢组学分析显示，棉酚治疗后糖异生前体谷氨酰胺浓度降低(P=0.047)，血浆赖氨酸、L苏氨酸和高丝氨酸水平显著降低(P<0.05)。棉酚组高密度脂蛋白胆固醇升高(P=0.044)，低密度脂蛋白胆固醇降低(P=0.023)。这些结果表明，棉酚可诱导奶牛氧化应激和肝脏毒性，降低外周脂肪代谢，促进肝脏脂肪堆积，降低氨基酸生物利用度和乳蛋白合成，减少乳糖异生和乳糖生成。
To characterize the metabolic disorders of dairy cows treated with gossypol, 12 dairy cows were assigned to either a control group or a treatment group that was fed 1000 mg of gossypol per kilogram of dry matter feed for 28 days. Milk quality was adversely affected, as both milk protein and lactose levels were significantly decreased in the gossypol-treated group (3.40% vs 3.16%,P= 0.044; 5.15% vs 4.91%,P= 0.027; respectively). Plasma samples revealed increases in alanine aminotransferase (P= 0.092), choline esterase (P= 0.02), and glutathione transferase (P= 0.0005) and decreases in glucose (P= 0.076) in the gossypol-treated group. Mass spectrometry based comparative metabolomic analyses showed reduced concentrations of the gluconeogenesis precursorl-glutamine (P= 0.047), with significant decreases (P< 0.05) in plasmal-lysine,l-threonine, and homoserine levels after gossypol treatment. HDL-C and LDL-C levels in the gossypol-treated group were increased (P= 0.044) and decreased (P= 0.023), respectively. These results demonstrate that gossypol induced oxidative stress and hepatotoxicity; reduced peripheral lipid metabolism, and enhanced hepatic lipid accumulation; decreased amino acid bioavailability and milk protein synthesis; and decreased gluconeogenesis and milk lactose in dairy cows.