MINICELLS OF BACILLUS-SUBTILIS
MINICELLS OF BACILLUS-SUBTILIS
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DOI：
10.1128/jb.114.2.860-873.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
COLE, RM
中科院分区：
文献类型：
--
作者：
REEVE, JN;MENDELSO.NH;COLE, RM
经亚硝基胍（N-甲基-N′-硝基-N-亚硝基胍）诱变后，分离到两株分裂位点沿细胞长度方向沿着分布有缺陷的枯草芽孢杆菌（Bacillus subtilismutants，IV-A1和IV-B1）。通过转化将这两个突变体转移到菌株CU 403中，并在CU 403遗传背景下研究它们的性质。在两种突变体中，靠近细胞极区的分裂位置导致小细胞产生。纯化的小细胞含有与亲本细胞中发现的核糖核酸与蛋白质的比率相当的核糖核酸与蛋白质的比率。~ 3 H-胸腺嘧啶掺入脱氧核糖核酸（DNA）的放射自显影、胸腺嘧啶-2-14C掺入DNA的放射自显影、电子显微镜和DNA的化学分析均不能显示小细胞中的DNA。由两种突变体产生的微细胞都是高度能动的，这是功能性能量代谢的指示。电子显微照片显示，微细胞产生的结构正常的分裂机制和微细胞包含一个正常的细胞表面。已通过PBS 1转导连锁toura定位IV-A1突变。IV-B1突变通过PBS 1转导和共转化与A紧密相连。
After nitrosoguanidine (N-methyl-N′-nitro-N-nitrosoguanidine) mutagenesis, twoBacillus subtilismutants (div IV-A1anddiv IV-B1) were isolated that are defective in the location of division site along cell length. Both mutations were transferred into strain CU403 by transformation, and their properties were studied in the CU403 genetic background. Location of divisions in close proximity to cell pole regions in both mutants results in minicell production. Purified minicells contain a ratio of ribonucleic acid to protein comparable to that found in the parent cells. Autoradiographs of3H-thymine incorporation into deoxyribonucleic acid (DNA), thymine-2-14Cincorporation into DNA, electron micrographs, and chemical analyses for DNA all fail to demonstrate DNA in the minicells. Minicells produced by both mutants are highly motile, an indication of functional energy metabolism. Electron micrographs reveal that minicells are produced by a structurally normal division mechanism and that minicells contain a normal cell surface. Thediv IV-A1mutation has been mapped by PBS1 transduction linked toura. Thediv IV-B1mutation is closely linked topheAby both PBS1 transduction and by co-transformation.