In vivo calibration of a subcutaneous glucose sensor for determination of subcutaneous glucose kinetics
In vivo calibration of a subcutaneous glucose sensor for determination of subcutaneous glucose kinetics
复制标题
用于测定皮下葡萄糖动力学的皮下葡萄糖传感器的体内校准
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
G. Reach
中科院分区：
文献类型：
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作者：
G. Velho;P. Froguel;D. Thévenot;G. Reach
这项工作的目的是开发一种体内校准皮下葡萄糖传感器的方法；该校准程序用于研究血糖水平变化期间估计的皮下葡萄糖浓度的动力学。这些实验在麻醉大鼠中进行：1) 在 25 只动物中，静脉注射 1 单位胰岛素后，血糖从 5.8 ± 0.2 降至 2.9 ± 0.2 mmol/1 平台。随后信号幅度下降了 5 m，从 13.54 ± 1.09 下降到 10.28 ± 1.05 nA (p < 0.01)； 2) 这两种稳态允许对传感器进行两点体内校准并计算随后的葡萄糖给药期间的表观皮下血糖水平： a) 连续葡萄糖输注 (n = 6) 在 35 分钟内将血糖升高至 5.6 ± 0.4 mmol/1 平台后，皮下血糖水平增加至 5.1 ± 0.2 mmol/1 平台 (NS)；当血糖升高至11.3 i 0.3 mmol/1 (n = 7)时，皮下葡萄糖浓度升高至10.7 ± 0.4 mmol/1 (NS)； b) 相反，葡萄糖推注后 (n = 7)，2 分钟时的血糖峰值为 12.4 ± 0.9 mmol/1，而 2 分钟时观察到的皮下葡萄糖峰值仅为 6.2 ± 0.6 mmol/1 (p < 0.01)。因此，我们得出结论，在稳态条件下，表观皮下葡萄糖浓度反映了血糖水平，从而验证了皮下组织作为用于闭环胰岛素输送系统的葡萄糖传感器的植入部位。
The aim of this work was to develop a method for calibrating in vivo a subcutaneous glucose sensor; this calibration procedure was used to investigate the kinetics of the estimated subcutaneous glucose concentration during variations in blood glucose level. These experiments were performed in anaesthetized rats: 1) In 25 animals, glycaemia was decreased from 5.8 ± 0.2 to a 2.9 ± 0.2 mmol/1 plateau, following the intravenous injection of 1 unit of insulin. This drop was followed by a decrease in the signal amplitude with a 5 m in time lag, from 13.54 ± 1.09 to 10.28 ± 1.05 nA (p < 0.01); 2) These two steady states allowed to perform a two-point in vivo calibration of the sensor and to calculate the apparent subcutaneous glucose level during subsequent glucose administration: a) Following a continuous glucose infusion (n = 6) that increased glycaemia within 35 min to a 5.6 ± 0.4 mmol/1 plateau, the subcutaneous glucose level increased to a 5.1 ± 0.2 mmol/1 plateau (NS); when glycaemia was increased to 11.3 i 0.3 mmol/1 (n = 7), subcutaneous glucose concentration raised to 10.7 ± 0.4 mmol/1 (NS); b) In contrast, after a glucose bolus (n = 7), the glycaemic peak at 2 min was 12.4 ± 0.9 mmol/1, while the subcutaneous glucose peak, observed at 2 min, was only 6.2 ± 0.6 mmol/1 (p < 0.01). We conclude, therefore, that under steady state conditions, the apparent subcutaneous glucose concentration reflects blood glucose level, thus validating the subcutaneous tissue as a site for the implantation of a glucose sensor to be used in a closed-loop insulin delivery system.