Rhizobium leguminosarum biovar viciae 3841, deficient in 27-hydroxyoctacosanoate-modified lipopolysaccharide, is impaired in desiccation tolerance, biofilm formation and motility.
Rhizobium leguminosarum biovar viciae 3841, deficient in 27-hydroxyoctacosanoate-modified lipopolysaccharide, is impaired in desiccation tolerance, biofilm formation and motility.
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豆根瘤菌生物变种 3841 缺乏 27-羟基二十二烷酸酯修饰的脂多糖，其干燥耐受性、生物膜形成和运动能力受损。
DOI：
10.1099/mic.0.025031-0
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Yost,ChristopherK
中科院分区：
文献类型：
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作者：
Vanderlinde,ElizabethM;Muszyński,Artur;Harrison,JoeJ;Koval,SusanF;Foreman,DallasL;Ceri,Howard;Kannenberg,ElmarL;Carlson,RussellW;Yost,ChristopherK
革兰氏阴性豆科共生根瘤菌Rhizobium leguminesculumbiovarviciae 3841的脂多糖（LPS）含有几种独特的修饰，包括在脂质A的2′位添加27-羟基二十八烷酸（27 OHC 28：0），也称为极长链脂肪酸（VLCFA）。 从VLCFA生物合成簇中分离并鉴定了一个转座子突变体，该突变体缺乏两个假定的3-氧代酰基[酰基载体蛋白]合酶II基因fabF 1和fabF 2的表达。MS表明突变体的脂质A缺乏VLCFA修饰，并且LPS的脱氧胆酸钠（DOC）-PAGE表明进一步的结构改变。该突变体的特点是敏感的几个应力，将在土壤环境中经历，如干燥和渗透胁迫。在突变体中观察到中性表面多糖的分泌增加。这种突变体也改变了其附着固体表面，是非能动的，与大多数的突变体细胞缺乏鞭毛。尽管这种突变具有多效性，但这些突变体仍然能够使豆科植物生长并固定大气中的氮。这份报告强调，一个结构完整的VLCFA含有脂质A是至关重要的细胞性状，是重要的生存在根际。
The lipopolysaccharide (LPS) of the Gram-negative legume symbiontRhizobium leguminosarumbiovarviciae3841 contains several unique modifications, including the addition of a 27-hydroxyoctacosanoic acid (27OHC28 : 0), also termed the very long chain fatty acid (VLCFA), attached at the 2′ position of lipid A. A transposon mutant that lacks expression of two putative 3-oxo-acyl [acyl-carrier protein] synthase II genes,fabF1andfabF2, from the VLCFA biosynthetic cluster, was isolated and characterized. MS indicated that the lipid A of the mutant lacked the VLCFA modification, and sodium deoxycholate (DOC)-PAGE of the LPS indicated further structural alterations. The mutant was characteristically sensitive to several stresses that would be experienced in the soil environment, such as desiccation and osmotic stresses. An increase in the excretion of neutral surface polysaccharideswas observed in the mutant. This mutant was also altered in its attachment to solid surfaces, and was non-motile, with most of the mutant cells lacking flagella. Despite the pleiotropic effects of the mutation, these mutants were still able to nodulate legumes and fix atmospheric nitrogen. This report emphasizes that a structurally intact VLCFA-containing lipid A is critical to cellular traits that are important for survival in the rhizosphere.