SINGLE VIBRISSAL CORTICAL COLUMN IN SI CORTEX OF RAT AND ITS ALTERATIONS IN NEONATAL AND ADULT VIBRISSA-DEAFFERENTED ANIMALS - A QUANTITATIVE 2DG STUDY
SINGLE VIBRISSAL CORTICAL COLUMN IN SI CORTEX OF RAT AND ITS ALTERATIONS IN NEONATAL AND ADULT VIBRISSA-DEAFFERENTED ANIMALS - A QUANTITATIVE 2DG STUDY
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DOI：
10.1152/jn.1988.60.2.829
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发表时间：
1988-08-01
影响因子：
2.5
通讯作者：
HAND, CL
中科院分区：
文献类型：
--
作者：
KOSSUT, M;HAND, PJ;HAND, CL
1.定量2-[14 C]脱氧-D-葡萄糖（2DG）放射自显影研究进行了清醒限制大鼠的第一体感皮层（SI）的个人列的功能（代谢）组织。手动或机械控制刷抚摸一个单一的触须（通常C3），以唤起神经活动，并增加葡萄糖利用率在一个单一的皮质桶在第四层和相邻的上和颗粒下层登记与单皮质桶标记在第四层。2.发现单个激活的触须柱从对侧SI皮质的层I延伸到层VI的浅半部，并且形状为梭形，在层IV中具有最大直径（650 μ m），在层VI中具有最小直径（200 μ m）。在层VI中。3.这个单一的代谢柱在其标记密度上是不均匀的。最大的局部脑葡萄糖代谢率（LCMRG）发现于IV层（170 μ mol·cm-1）。100 g-1. min-1），层II-III和V平均具有相似的LCMRG值（115 μ mol·cm-1）。100 g-1. min ~（-1），随距离增加而减小。4.功能（代谢）柱状活动中心位于第四层对应的大小，以适当的解剖桶确定从硫堇染色切片。代谢活动的增加并不严格局限于蜡烛针形的圆柱，而是作为辐射状的指状突起向邻近的桶切向延伸。5.在皮质层以外的IV，代谢列不太突出的标记，和代谢活动也分支的切平面。上层的标记模式的特点是大量的横向扩展的指状突起的葡萄糖代谢增加。6.新生儿卵泡病变仅保留C3触须导致皮质领域的巨大增加（125%），由备用晶须驱动，伴随着柱状形状的损失和代谢活动的强度降低，以响应其刺激。7.成年大鼠的类似损伤使备用C3皮质柱的尺寸显着增加（40%），但小于新生儿损伤后产生的尺寸。与对照柱相比，备用C3柱中Vb层的代谢活化增加（17%）。8.在刺激C3触须后在大鼠的S1皮质中观察到的代谢活性增加的单个柱，由于其局部性质和不同皮质层内其标记的密度、面积范围和模式的异质性，一个很好的模型，用于研究躯体感觉信息的正常处理以及后内侧桶子区（PMBSF）内传入神经阻滞产生的代谢可塑性和大脑皮层。
1. A quantitative 2-[14C]deoxy-D-glucose (2DG) autoradiographic study was undertaken to determine the functional (metabolic) organization of an individual column in the first somatosensory cortex (SI) of the awake restrained rat. Manual or mechanically controlled brush stroking of a single vibrissa (typically C3) was used in order to evoke neural activity and increase glucose utilization in a single cortical barrel in lamina IV and in adjacent supra- and infragranular layers in register with the single cortical barrel labeling in lamina IV. 2. The single activated vibrissal column was found to extend from layer I to the superficial half of layer VI of the contralateral SI cortex and was fusiform in shape, being of largest diameter (650-.mu.m) in layer IV and smallest diameter (200 .mu.) in layer VI. 3. This single metabolic column was not uniform in its density of labeling. The greatest local cerebral metabolic rate of glucose (LCMRG) was found in layer IV (170 .mu.mol .cntdot. 100 g-1 .cntdot. min-1), layers II-III, and V had, on the average, similar LCMRG values (115 .mu.mol .cntdot. 100 g-1 .cntdot. min-1), which decreased with distance from layer IV. 4. The functional (metabolic) columnar activity center situated in layer IV corresponded in size to the appropriate anatomical barrel as determined from thionin-stained sections. Increased metabolic activity was not strictly confined to the candle-pin-shaped column but extended tangentially as radiating fingerlike projections toward the neighboring barrels. 5. In cortical layers other than IV, the metabolic column was less prominently labeled, and the metabolic activity also branched in the tangential plane. The labeling pattern profile in upper layers was characterized by numerous lateral spreading fingerlike projections of increased glucose metabolism. 6. Neonatal follicle lesions sparing only C3 vibrissa caused a great increase (125%) in cortical territory driven by the spared whisker with a concomitant loss of columnar shape and decrease in intensity of metabolic activity in response to its stimulation. 7. Similar lesions in adult rats produced a significant increase (40%) in dimensions of the spared C3 cortical column, which was, however, smaller than that produced after neonatal lesions. The metabolic activation of layer Vb in the spared C3 column was increased (17%) compared with that of the control column. 8. The single column of increased metabolic activity observed in SI cortex of the rat after stimulation of C3 vibrissa provides, because of its localized nature and the heterogeneity in the density, areal extent, and pattern of its labeling within the different cortical laminae, a good model for investigating normal processing of somatosensory information as well as deafferentation-produced metabolic plasticity within the posteromedial barrel subfield (PMBSF) and neocortex, in general.