Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana
Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana
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DOI：
10.1007/s00425-006-0261-9
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发表时间：
2006-09-01
期刊：
影响因子：
4.3
通讯作者：
Pelloux, Jerome
中科院分区：
文献类型：
--
作者：
Louvet, Romain;Cavel, Emilie;Pelloux, Jerome
果胶甲酯酶（PME，EC. 3.1.1.11）是在Muro中使植物细胞壁果胶脱甲基的酶。在拟南芥中，推定的PME蛋白被认为是由66个成员的基因家族编码。本研究利用实时荧光定量RT-PCR技术对拟南芥角果发育的8个阶段、花芽和营养组织中整个家族的表达进行了概述。只有15%的PMEs在研究的任何发育阶段都不表达。在表达的PMEs中，表达数据可以被聚类成五个不同的组：19个在花芽中高度或独特表达的PMEs，4个在角果发育中期独特表达的PMEs，16个在角果发育后期高度或独特表达的PMEs，16个PMEs大多是普遍表达的，以及1个具有特定表达模式的PMEs，即在角果发育早期不表达。表达和系统发育谱的比较表明，在系统发育组2，所有，但一个PME属于花芽表达组。类似的结果显示了一个子集的一个系统发育组，它不同于其他的包含大多数的PMEs不具有任何PRO部分旁边的催化部分。通过两个启动子证实了表达数据：GUS转基因植物分析揭示了在花粉中表达的PME和在幼种子中表达的PME。我们的研究结果突出了PME表达谱的高度多样性。他们讨论方面的作用PMEs在果实发育和细胞生长。
Pectin methylesterases (PME, EC. 3.1.1.11) are enzymes that demethylesterify plant cell wall pectins in muro. In Arabidopsis thaliana, putative PME proteins are thought to be encoded by a 66-member gene family. This study used real-time RT-PCR to gain an overview of the expression of the entire family at eight silique developmental stages, in flower buds and in vegetative tissue in the Arabidopsis. Only 15% of the PMEs were not expressed at any of the developmental stages studied. Among expressed PMEs, expression data could be clustered into five distinct groups: 19 PMEs highly or uniquely expressed in floral buds, 4 PMEs uniquely expressed at mid-silique developmental stages, 16 PMEs highly or uniquely expressed in silique at late developmental stages, 16 PMEs mostly ubiquitously expressed, and 1 PME with a specific expression pattern, i.e. not expressed during early silique development. Comparison of expression and phylogenetic profiles showed that, within phylogenetic group 2, all but one PME belong to the floral bud expression group. Similar results were shown for a subset of one of the phylogenetic group, which differed from others by containing most of the PMEs that do not possess any PRO part next to their catalytic part. Expression data were confirmed by two promoter:GUS transgenic plant analysis revealing a PME expressed in pollen and one in young seeds. Our results highlight the high diversity of PME expression profiles. They are discussed with regard to the role of PMEs in fruit development and cell growth.