Genetic similarity among Arabidopsis thaliana ecotypes estimated by DNA sequence comparison
Genetic similarity among Arabidopsis thaliana ecotypes estimated by DNA sequence comparison
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DOI：
10.1007/bf00020488
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发表时间：
1996-12
影响因子：
5.1
通讯作者：
C. Hardtke;Judith M. Müller;T. Berleth
中科院分区：
文献类型：
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作者：
C. Hardtke;Judith M. Müller;T. Berleth
拟南芥的DNA多态在基于图谱的克隆策略中被广泛用作遗传标记。新的基于聚合酶链式反应的分子标记不仅有助于分子作图，而且还可以用于获得可靠的序列信息，用于分支分析。我们使用CAPS(裂解扩增多态序列)标记和直接测序策略来估计18个拟南芥亚型之间的遗传相似性。对四个基因座的序列进行了分析，其中两个来自核基因组，两个来自非核基因组。对于每个生态型，获得了超过1000pb的序列信息，并使用基于特征的方法计算了总共35个多态位的遗传相似性。在由多态性定义的72个性状中，差异从0到50个不协调的字符不等。根据核序列和非核序列进行了单独的计算，发现了一些共同特征，包括存在因广泛的序列差异而彼此分离的非常密切相关的生态型的小簇。我们的结果提供了特别有用的信息，特别是对于为染色体着陆策略设置杂交的研究人员。
DNA polymorphisms amongArabidopsis thalianaecotypes are widely used as genetic markers in map-based cloning strategies. New PCR-based molecular markers do not only facilitate molecular mapping, but can also be used to obtain reliable sequence information for cladistic analyses. We have used CAPS (cleaved amplified polymorphic sequences) markers and a direct sequencing strategy to estimate genetic similarity among eighteenArabidopsisecotypes. Sequences at four loci, two from the nuclear and two from a non-nuclaar genome, were analysed. For each ecotype more than 1000pb of sequence information was obtained, and genetic similarity was calculated from a total of 35 polymorphic sites using a character-based approach. Divergence ranged from zero up to 50 discordant characters among the 72 characters defined by the polymorphisms. Separate calculations based on the nuclear and the non-nuclear sequences were performed and revealed a number of common features, including the existence of small clusters of very closely related ecotypes separated from each other by extensive sequence divergence. Our results provide information useful especially to investigators setting up crosses for chromosome landing strategies.