Quantitative in vitro transformation of Syrian golden hamster embryo cells with the use of frozen stored cells.
Quantitative in vitro transformation of Syrian golden hamster embryo cells with the use of frozen stored cells.
复制标题
使用冷冻储存细胞对叙利亚金仓鼠胚胎细胞进行体外定量转化。
DOI：
--
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. DiPaolo
中科院分区：
文献类型：
--
作者：
J. DiPaolo
叙利亚金黄地鼠胚胎细胞的致癌物的定量转化，从初级，二级或三级培养物可以获得从细胞池冷冻前任何培养与新鲜细胞相同的效率。当未培养的仓鼠细胞以受控的速度冷却时，并且当遵循既定的细胞培养程序时，细胞保留了激活广泛的化学致癌物的能力。用仓鼠细胞系替代饲养层，可以增加用冷冻仓鼠池细胞进行的实验数量。不同类别的化学致癌物，包括致癌碳氢化合物，芳香胺衍生物，金属络合物，黄曲霉毒素B1，N-甲基-N '-硝基-N-亚硝基胍，和紫外线照射的反应是类似的新鲜细胞报告。转化率随着致癌物浓度的增加而增加。列举了对健康动物的繁殖和选择适合于获得可重复转化实验的因素的重要性。
Quantitative transformation by carcinogens of Syrian golden hamster embryo cells from primary, secondary, or tertiary cultures could be obtained from cell pools frozen prior to any culturing with the same efficiency as with fresh cells. Cells retained the ability to activate a wide range of chemical carcinogens when the noncultured hamster cells were cooled at a controlled rate and when the established procedures for culturing cells were followed. The number of experiments that could be done with cells derived from frozen hamster pools could be increased by substitution of a hamster cell line for the feeder layer. The response to chemical carcinogens of different classes including carcinogenic hydrocarbons, aromatic amine derivatives, metal complexes, aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine, and UV irradiation was similar to that reported with fresh cells. Transformation rate increased with increasing carcinogen concentration. The factors important for the breeding of healthy animals and for selection of those appropriate for obtaining reproducible transformation experiments were enumerated.