OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice
OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice
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DOI：
10.1111/nph.14941
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发表时间：
2018-02-01
期刊：
影响因子：
9.4
通讯作者：
Nishizawa, Yoko
中科院分区：
文献类型：
--
作者：
Desaki, Yoshitake;Kouzai, Yusuke;Nishizawa, Yoko
植物细胞表面受体样激酶（RLKs）介导微生物相关分子模式（MAMPs）信号，诱导免疫应答。脂多糖（Lipopolysaccharide，LPS）是革兰氏阴性菌外膜的主要成分，是动植物常见的MAMP;然而，除了拟南芥中的球茎型凝集素S-结构域RLK（B-lectin SD 1-RLK）LORE外，植物受体/共受体尚不清楚。OsCERK 1是水稻中的多功能RLK，其含有溶素基序（LysMs），并且对于几丁质（真菌MAMP）和肽聚糖（细菌MAMP）的感知是必需的。本研究分析了OsCERK 1基因与水稻感受LPS的相关性，并利用OsCERK 1基因敲除突变体（oserk 1）研究了LPS处理后水稻过氧化氢（H2 O2）的产生和基因表达。我们还研究了水稻B-凝集素SD 1-RLK基因敲除突变体和拟南芥LysM蛋白基因敲除突变体的LPS反应，与野生型水稻细胞相比，oserk 1细胞的LPS反应大部分减弱。与此相反，B-lectin SD 1-RLK基因突变的水稻品系和LysM蛋白基因突变的拟南芥品系对LPS的反应正常，表明OsCERK 1是一个LPS受体/辅助受体，水稻和拟南芥的LPS感受系统存在显著差异。
Plant cell surface receptor-like kinases (RLKs) mediate the signals from microbe-associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram-negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for LORE, a bulb-type lectin S-domain RLK (B-lectin SD1-RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP. Here, we analyzed the relevance of OsCERK1 to LPS perception in rice.Using OsCERK1-knockout mutants (oscerk1), we evaluated hydrogen peroxide (H2O2) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B-lectin SD1-RLK genes in rice and for all LysM-protein genes in Arabidopsis.Compared with wild-type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B-lectin SD1-RLK genes and Arabidopsis lines mutated in the LysM-protein genes responded normally to LPS.From these results, we conclude that OsCERK1 is an LPS receptor/co-receptor and that the LPS perception systems of rice and Arabidopsis are significantly different.