RADIOIMMUNOASSAY OF PLASMA DEHYDROEPIANDROSTERONE SULFATE
RADIOIMMUNOASSAY OF PLASMA DEHYDROEPIANDROSTERONE SULFATE
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DOI：
10.1080/00032717208062119
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发表时间：
1972-01-01
影响因子：
2
通讯作者：
ABRAHAM, GE
中科院分区：
文献类型：
--
作者：
BUSTER, JE;ABRAHAM, GE
本文报道了直接测定稀释血浆中硫酸脱氢表雄酮(脱氢表雄酮-S)的放射免疫分析方法。利用一种与脱氢表雄酮完全反应的特异性抗血清，我们准确地测定了小等份血浆中脱氢表雄酮-S。由于脱氢表雄酮-S在血浆中的浓度较高，脱氢表雄酮和其他类固醇对测定的影响可以忽略不计。标准曲线可用范围为10~500pg DHEA-S。每毫升血浆可以测量的浓度范围从0.02到5.0pg不等。用这种方法得到的血浆脱氢表雄酮-S的值范围与使用各种其他技术在成年男性和女性身上观察到的水平范围相比较是有利的。
This report describes a radioimmunoassay method for the direct measurement of dehydroepiandrosterone sulfate (DHEA-S) in diluted plasma. Utilizing a specific DHEA-S antiserum which reacted completely with DHEA, we have measured DHEA-S accurately in small aliquots of diluted plasma. Due to the relatively high concentration of DHEA-S in plasma, the effect of DHEA and other steroids on the assay was negligible. The usable range of the standard curve was between 10 and 500 pg DHEA-S. The range of levels that could be measured per ml of plasma varied from 0.02 to 5.0 pg. The range of values obtained by this method for plasma DHEA-S compared favorably with the ronge of levels observed in adult men and women, using various other techniques.