Impact of cytogenetic and genomic aberrations of the kallikrein locus in ovarian cancer
Impact of cytogenetic and genomic aberrations of the kallikrein locus in ovarian cancer
复制标题
DOI：
10.1016/j.molonc.2008.07.001
复制
发表时间：
2008-10-01
影响因子：
6.6
通讯作者：
Diamandis, Eleftherios P.
中科院分区：
文献类型：
--
作者：
Bayani, Jane;Paliouras, Miltiadis;Diamandis, Eleftherios P.
组织激肽素（KLK）基因是卵巢癌生物标志物的新来源。然而，目前还没有系统的分析拷贝数和结构重排与它们的蛋白表达有关。用FISH研究KLK区域的染色体重排和拷贝数变化，用ELISA检测蛋白水平。卵巢癌和细胞系显示，KLK区域受拷贝数不平衡或参与不平衡易位，并与klk5、6、7、8、9、10和11蛋白表达增加有关。在这项初步研究中，我们引入了远程染色体效应和拷贝数作为先前报道的许多KLK基因在卵巢癌中异常表达的机制。(C) 2008年欧洲生化学会联合会出版，Elsevier B.V.版权所有
The tissue kallikrein (KLK) genes are a new source for biomarkers in ovarian cancer. However, there has been no systematic analysis of copy number and structural rearrangements related to their protein expression. Chromosomal rearrangements and copy number changes of the KLK region were studied by FISH with protein levels measured by ELISA. Ovarian cancer and cell lines revealed the KLK region was subject to copy number imbalances or involved in unbalanced translocations and were associated with increased protein expression of KLKs 5, 6, 7, 8, 9, 10 and 11. in this initial study, we introduce the potential for long-range chromosomal effects and copy number as a mechanism for the previously reported aberrant expression of many KLK genes in ovarian cancers. (C) 2008 Federation of European Biochemical Societies Published by Elsevier B.V. All rights reserved