Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation
Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation
复制标题
DOI：
10.1152/ajpregu.00735.2004
复制
发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Schwartz, GJ
中科院分区：
文献类型：
--
作者：
Purkerson, JM;Schwartz, GJ
几种碳酸酐酶（CA）同工型与质膜相关。这些酶可能与阴离子转运蛋白相互作用，以促进HCO 3-进出细胞。更好地了解CA亚型在给定组织中的表达将有助于系统检查与这些转运蛋白的任何关联。我们使用实时定量逆转录聚合酶链反应（RT-PCR）检测了CAs IV、IX、XII和XIV mRNA在兔组织（包括肾脏、心脏、肺、骨骼肌、肝脏、胰腺、胆囊、胃、小肠、结肠和脾脏）中的表达。CA Ⅳ mRNA主要分布在肾脏、心脏、肺、结肠和胆囊。CA IX mRNA仅限于胃、胆囊、十二指肠和早期空肠。CA XII mRNA在肾脏和结肠中发现。CA XIV mRNA定位于心脏、肺、骨骼肌和肝脏。这些数据表明，在各种组织中有不同的CA表达模式：CA IX在近端胃肠道中表达，而CA XII和CA IV在更远端。CA IV和CA XII是重要的肾脏亚型。CA XIV在代谢活跃的组织如肝、心、肺和骨骼肌中含量丰富。在这些亚型中的一些亚型的表达中注意到一些显著的种属差异;例如，CA XIV在兔肾脏中不表达，尽管在小鼠肾脏中丰富。成熟期研究表明，CA IX mRNA和蛋白的表达随断奶（与生后3 ~ 4周相似）而显著增加，并与胃H ~+，K ~+-ATP酶α亚基的成熟期表达密切相关，提示CA IX与胃H ~+泵的功能在成年期食物消化中可能存在联系。膜结合CA亚型的独特模式表明与转运蛋白的不同功能关联，取决于对组织的生理需求。
Several carbonic anhydrase ( CA) isoforms are associated with plasma membranes. It is probable that these enzymes interact with anion transporters to facilitate the movement of HCO3- into or out of the cell. A better knowledge of CA isoform expression in a given tissue would facilitate a systematic examination of any associations with such transporters. We examined the expression of CAs IV, IX, XII, and XIV mRNAs in rabbit tissues, including kidney, heart, lung, skeletal muscle, liver, pancreas, gall bladder, stomach, small intestine, colon, and spleen, using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). CA IV mRNA was mainly in kidney, heart, lung, colon, and gall bladder. CA IX mRNA was restricted to stomach, gall bladder, duodenum, and early jejunum. CA XII mRNA was found in kidney and colon. CA XIV mRNA was localized to heart, lung, skeletal muscle, and liver. The data indicate that there are different patterns of CA expression in various tissues: CA IX was expressed in the proximal gastrointestinal tract, whereas CA XII and CA IV were more distal. CA IV and CA XII are important kidney isoforms. CA XIV was abundant in metabolically active tissues such as liver, heart, lung, and skeletal muscle. Some significant species differences were noted in the expression of some of these isoforms; for example, CA XIV is not expressed in rabbit kidney, despite being abundant in mouse kidney. Maturational studies showed that the expression of CA IX mRNA and protein increased markedly with weaning ( similar to 3 - 4 postnatal wk) and was well correlated with the maturational expression of the alpha-subunit of the gastric H+, K+-ATPase, suggesting that function of CA IX and the gastric H+ pump might be linked in the digestion of adult foodstuffs. The unique pattern of membrane-bound CA isoforms suggests different functional associations with transporters, depending on the physiological demands on the tissue.