Transcriptional and translational control of the message for transition protein 1, a major chromosomal protein of mammalian spermatids.
Transcriptional and translational control of the message for transition protein 1, a major chromosomal protein of mammalian spermatids.
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DOI：
10.1016/s0021-9258(18)45202-7
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发表时间：
1987-09
期刊：
影响因子：
--
通讯作者：
M. Heidaran;W. Kistler
中科院分区：
文献类型：
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作者：
M. Heidaran;W. Kistler
精子细胞过渡蛋白包括一组基本的染色体蛋白，其出现在精子细胞经历核伸长和浓缩的时期，大约在减数分裂结束和精子从曲细精管释放之间的中间。这些过渡蛋白取代组蛋白，但它们本身随后被鱼精蛋白取代，它们在精子核中没有发现。我们已经使用了一个cDNA克隆的最小的过渡蛋白（TP 1，54个氨基酸），以表明它的消息首先出现在减数分裂后期圆形精子细胞。因此，TP 1的产生是单倍体基因表达的一个实例。在早期伸长的精子细胞中，在翻译发生之前，该信息保持不活跃约3-4天。虽然TP 1的信号被抑制，但它是非多聚核糖体的，具有约590个碱基的离散大小，包括一个140个残基的poly（A）尾。相比之下，由于poly（A）长度的可变性，多聚体相关信息具有异质大小。
The spermatid transition proteins comprise a set of basic chromosomal proteins that appear during the period when spermatids are undergoing nuclear elongation and condensation, about midway between the end of meiosis and the release of spermatozoa from the seminiferous tubule. The transition proteins replace the histones but are themselves subsequently replaced by protamines, and they are not found in sperm nuclei. We have used a cDNA clone for the smallest transition protein (TP1, 54 amino acids) to show that its message first appears postmeiotically in late round spermatids. Thus production of TP1 is an example of haploid gene expression. The message remains translationally inactive for some 3-4 days before translation occurs in early elongating spermatids. While translationally repressed, TP1 message is nonpolysomal and has a discrete size of about 590 bases, including a 140 residue poly(A) tail. In contrast, polysome-associated message is of heterogeneous size due to variability of poly(A) lengths.