Effects of sugars on the kinetics of drying and on the survival of partially dehydrated larvae of Anopheles mosquitoes
Effects of sugars on the kinetics of drying and on the survival of partially dehydrated larvae of Anopheles mosquitoes
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DOI：
10.1016/s0022-1910(03)00070-2
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发表时间：
2003-07-01
影响因子：
2.2
通讯作者：
Mazur, P
中科院分区：
文献类型：
--
作者：
Liu, XH;Mazur, P
将某一阶段的按蚊冷冻保存的能力将有助于发展出不能传播疟疾的品系。超低温保存要求细胞系统中的可冻结水被移除或使其不能形成冰。本文研究了冈比亚按蚊1龄幼虫风干脱水的速率、幼虫可耐受的脱水程度以及海藻糖和蔗糖对干燥动力学和存活率的影响。81%的幼虫是水。风干可以在大约20分钟内除去90%的水分。如果用1/2倍等渗盐水(0.13OSM)复水，幼虫部分脱水后的存活率最高；如果用水或0.13OSM蔗糖复水，则存活率最低。在前者，大约34%的人在去除一半的水后存活下来，但几乎没有人在失去70%的初始水后存活下来。预先暴露在0.2M海藻糖中只需1分钟，就可以减缓干燥速度，并增加幼虫对脱水的耐受性。暴露30分钟后，88%的人能在50%的水分损失中存活下来，63%的人能在75%的损失中存活下来。0.4M海藻糖可消除保护作用。这一结果与蔗糖相似。如果暴露在糖中的幼虫在干燥前用水短暂清洗，它会大大减少。这种保护似乎与干燥速度的降低无关。相反，它看起来是相关的。通过一种未知的机制，导致幼虫外表面存在糖。(C)2003爱思唯尔科学有限公司。保留所有权利。
The ability to cryopreserve a stage of Anopheles mosquitoes would facilitate the development of strains incapable of transmitting malaria. Cryopreservation requires that the freezable water in cell systems be removed or rendered incapable of undergoing ice formation. The present study was concerned with the rate at which water is removed from 1st instar larvae of Anopheles gambiae by air-drying, with the extent of dehydration that the larvae will tolerate, and with the effect of trehalose and sucrose on both drying kinetics and survival. Eighty-one percent of the larvae are water. Air-drying removes 90% of that water in similar to20 min. Survivals after partial dehydration are highest if the larvae are rehydrated in 1/2x isotonic saline (0.13 osm); they are poorest if rehydrated in water or 0.13 osm sucrose. In the former, about 34% survive the removal of half the water, but next to none survive the loss of >70% initial water. Prior exposure to 0.2 M trehalose for as little as 1 min slows the drying rate and increases the tolerance of the larvae to dehydration. With 30-min exposure, 88% survive the loss of 50% of their water and 63% survive the loss of 75%. Protection is abolished with 0.4 M trehalose. The results are similar with sucrose. It is substantially reduced if sugar-exposed larvae are briefly washed with water prior to drying. The protection appears not to be related to the decreased drying rate. Rather it appears related. by an unknown mechanism, to the presence of sugar on the outer surface of the larvae. (C) 2003 Elsevier Science Ltd. All rights reserved.