Immunisation with a plasmid DNA vaccine encoding gonadotrophin releasing hormone (GnRH-1) and T-helper epitopes in saline suppresses rodent fertility
Immunisation with a plasmid DNA vaccine encoding gonadotrophin releasing hormone (GnRH-1) and T-helper epitopes in saline suppresses rodent fertility
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DOI：
10.1016/j.vaccine.2007.12.052
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发表时间：
2008-03-04
期刊：
影响因子：
5.5
通讯作者：
Kimura, Tadashi
中科院分区：
文献类型：
--
作者：
Khan, Mohammad A. H.;Ogita, Kazuhide;Kimura, Tadashi
针对促性腺激素释放激素（GnRH-1）的主动免疫研究已被广泛接受，作为控制农场、伴侣和野生动物繁殖和行为的手段。许多研究描述了使用自肽的多个拷贝线性排列并与大型载体蛋白结合以增加对肽的免疫反应。然而，由载体蛋白表位抑制引起的问题已经转移到使用遗传物质来引发最佳免疫反应。本研究以pcDNAV5-HisB编码18.871 kDa的GnRH-I-T-heLper-V5表位融合蛋白，构建了533 bp长的DNA疫苗。转染疫苗构建体的COS1细胞释放融合蛋白到培养上清中。该疫苗构建物（1100 μ g/只）在生理盐水中注入ICR雄性和雌性小鼠的前股四头肌，刺激抗原特异性IgG抗体反应。接种疫苗的雄性小鼠的睾酮水平显著降低（p = 0.021）。接种疫苗的雌性与对照组交配后，子宫植入物显著减少（p=0.028），接种疫苗的雌性与对照组交配后，子宫植入物显著减少（p= 0.004）。研究第13周，雄性和雌性性腺的组织学检查显示精细胞上皮萎缩，卵泡发生抑制。(C) 2008 Elsevier Ltd版权所有。
Research into active immunisation against gonadotrophin releasing hormone (GnRH-1) has gained widespread acceptance as a means of controlling reproduction and behaviour of farm, companion and wild animals. Many studies describe the use of muttipLe copies of the self-peptide in linear alignment and conjugation with a large carrier protein to increase the immune response to the peptide. However, problems resulting from carrier protein epitope suppression have seen a diversion of interest into the use of genetic materials to elicit an optimum immune response. In this study, a 533-bp Long DNA vaccine was constructed in pcDNAV5-HisB coding for 18.871 kDa GnRH-I-T-heLper-V5 epitopes fusion protein. COS1 cells transfected with the vaccine construct were found to release fusion protein into culture supernatant. The vaccine construct (1100 mu g/mice) in saline solution administered into the anterior quadriceps muscle of ICR mate and female mice stimulated antigen-specific IgG antibody responses. Testosterone levels in the vaccinated mate mice were significantly (p = 0.021) reduced. A significant reduction in uterine implants were noted following mating between immunised mates and control females (p=0.028), as well as between immunised females and control, mates (p = 0.004). Histotogical examination of both the mate and female gonads in study week 13 showed atrophy of the seminiferous epithelium and suppression of folliculogenesis. (C) 2008 Elsevier Ltd. All rights reserved.