Diversity of Ca2+-induced morphology revealed by morphological phenotyping of Ca2+-sensitive mutants of Saccharomyces cerevisiae
Diversity of Ca2+-induced morphology revealed by morphological phenotyping of Ca2+-sensitive mutants of Saccharomyces cerevisiae
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DOI：
10.1128/ec.00012-07
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发表时间：
2007-05-01
期刊：
影响因子：
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通讯作者：
Ohya, Yoshikazu
中科院分区：
文献类型：
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作者：
Ohnuki, Shinsuke;Nogami, Satoru;Ohya, Yoshikazu
利用图像处理软件CalMorph，酵母细胞形态可以作为一个数量性状来处理。在本研究中，我们研究了Ca~(2+)诱导的酿酒酵母突变株的形态变化，发现Ca~(2+)敏感突变体zds1在Ca~(2+)诱导下的形态变化反映了Ca~(2+)信号介导的细胞周期控制通路的变化。通过对58个cls突变体的数量形态数据进行系统聚类分析，根据形态相似性将其中31个突变体分为7类。同一类钙离子诱导的形态变化模式与另一类不同。基于多种表型分析方法得到的结果，我们得出结论：高浓度的钙离子对酵母产生多种不同的影响，并且存在多种不同于Zds1p相关的钙调节途径。
Yeast cell morphology can be treated as a quantitative trait using the image processing software CalMorph. In the present study, we investigated Ca2+-induced morphological changes in Ca2+-sensitive (cls) mutants of Saccharomyces cerevisiae, based on the discovery that the characteristic Ca2+-induced morphological changes in the Ca2+-sensitive mutant zds1 reflect changes in the Ca2+ signaling-mediated cell cycle control pathway. By applying hierarchical cluster analysis to the quantitative morphological data of 58 cls mutants, 31 of these mutants were classified into seven classes based on morphological similarities. The patterns of morphological change induced by Ca2+ in one class differed from those of another class. Based on the results obtained using versatile methods for phenotypic analysis, we conclude that a high concentration of Ca2+ exerts a wide variety of effects on yeast and that there are multiple Ca2+-regulatory pathways that are distinct from the Zds1p-related pathway.