Determination of compound kill kinetics against Mycobacterium tuberculosis.
Determination of compound kill kinetics against Mycobacterium tuberculosis.
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DOI：
10.1007/978-1-4939-2450-9_16
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Alling, Torey
中科院分区：
文献类型：
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作者：
Early, Julie;Alling, Torey
在这一章中，我们描述了如何确定一种化合物对结核分枝杆菌的杀灭动力学和最小杀菌浓度（MBC）。描述了三种条件下的技术：积极生长的好氧细菌，和非复制的细菌诱导的营养饥饿和/或低pH值。每种技术涉及确定在几个浓度的化合物的存在下，超过3周的活菌数。提供了如何解释结果、确定生长抑制化合物是杀菌还是抑菌以及化合物是否表现出时间依赖性或浓度依赖性杀灭的指南。
In this chapter, we describe how to determine the kill kinetics and minimum bactericidal concentration (MBC) of a compound against Mycobacterium tuberculosis. Techniques are described for three conditions: actively growing aerobic bacteria, and non-replicating bacteria induced by nutrient starvation and/or low pH. Each technique involves determining the number of viable bacteria in the presence of several concentrations of compound over 3 weeks. Guidelines for how to interpret the results, to determine if growth-inhibitory compounds are bactericidal or bacteriostatic and also whether compounds exhibit time-dependent or concentration-dependent kill are provided.