Interactions of Leptospira with Environmental Bacteria from Surface Water
Interactions of Leptospira with Environmental Bacteria from Surface Water
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DOI：
10.1007/s00284-011-9931-3
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发表时间：
2011-06-01
影响因子：
2.6
通讯作者：
Trueba, Gabriel A.
中科院分区：
文献类型：
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作者：
Barragan, Veronica A.;Eugenia Mejia, Maria;Trueba, Gabriel A.
钩端螺旋体在传播给哺乳动物宿主之前可以在营养不良的水环境中持续数月。与环境细菌的相互作用和生物膜的形成是钩端螺旋体在环境中持续存在的可能机制。从厄瓜多尔雨林的河流中分离出的细菌被测试其支持钩端螺旋体生存能力的能力。我们发现与鞘氨醇单胞菌属的共培养，而不是黄杆菌属。或Delftia属，使L得以生存。biflexa和L.在蒸馏水中浸泡一年我们还发现L.在蒸馏水中形成的问号线虫生物膜含有在血清或EMJH培养基中存在营养物的情况下迅速分散到反渗透相中的活生物体。这些数据告诉我们的理解钩端螺旋体的生存策略，使长期持续在营养不良的条件下，但允许快速动员时，营养变得可用。
Leptospires can persist for months in nutrient-poor aqueous environments prior to transmission to a mammalian host. Interactions with environmental bacteria and biofilm formation are possible mechanisms of persistence of leptospires in the environment. Bacteria isolated from rivers in the Ecuadorian rainforest were tested for their ability to support leptospiral viability. We found that co-culture with Sphingomonas spp., but not Flavobacterium spp. or Delftia spp., enabled survival of L. biflexa and L. meyeri for up to a year in distilled water. We also found that L. interrogans biofilms formed in distilled water contained viable organisms that rapidly dispersed into the planktonic phase in the presence of nutrients in serum or EMJH medium. These data inform our understanding of leptospiral survival strategies that enable long-term persistence in nutrient-poor conditions yet allow rapid mobilization when nutrients become available.